Spatial analyses encompass a wide scope of techniques for analysing, calculating, and representing the spatial data. The goal of the diploma thesis was to design a renewed manual for such analyses in ArcGIS tool. We have therefore referred to the existing manual (Bobnar, 2005), that we adjusted especially due to the technological changes and changes in content of the functioning of ArcGIS tool. The content is divided into a theoretical and practical part. In the first part, we present the spatial analyses in the geographical information systems (GIS). Firstly, the spatial analyses and the analyses of spatial data are defined, furthermore, the basic approaches to spatial analyses are presented. The thesis discusses in more detail the functional approach of spatial analyses classification. The second, practical part contains the so-called manual for spatial analyses in ArcGIS tool. Besides the renewed procedures for spatial analyses, the procedures in the field of network analyses in GIS environment are also added to the manual. The manual consists of ten exercises, which are performed in ArcGIS 10.2 and later versions. This software solution offers a wide range of tools to perform the spatial analyses. The user learns about the tools to launch the spatial analyses, calculate distances, perform network analyses and about the raster-based data layers and tools for their compilation. The manual also discusses the various techniques for surface interpolation, map algebra, cartographic modelling, and presents a graphical environment ModelBuilder for cartographic modelling.