Verticillium albo-atrum partitivirus 1 Is a highly prevalent and stably maintained cryptic virus of hop-associated Verticillium nonalfalfae

Miljanić, Vanja (avtor), Jakše, Jernej (avtor), Radišek, Sebastjan (avtor), Stajič, Ester (avtor), Štajner, Nataša (avtor)

The soilborne plant-pathogenic fungus Verticillium nonalfalfae causes Verticillium wilt in hop. Infections caused by its highly virulent (lethal) pathotype can lead to the development of a severe level of disease that poses a major threat to hop production. In this study, we investigated the occurrence of mycoviruses in V. nonalfalfae isolates representing different geographical regions and virulence groups using double-stranded RNA isolation and high-throughput sequencing. Notably, Verticillium albo-atrum partitivirus 1 (VaaPV1) was detected in 46 of 90 isolates (51.1%), including both lethal and mild pathotypes. Thirty isolates were sequenced, revealing low genetic divergence, with nucleotide identities of 93.9%–100% for RdRp and 94.5%–100% for CP. The low dN/dS ratio of the RdRp gene (0.119) further indicated strong purifying selection. VaaPV1 was efficiently maintained during fungal growth: hyphal tipping failed to eliminate the virus from any of 70 regenerated cultures, while only 13 of 73 cultures regenerated from protoplasts were virus-free. Furthermore, VaaPV1 was transmitted horizontally through hyphal anastomosis from all tested donor isolates. Comparisons between infected and isogenic virus-free isolates revealed no significant differences in fungal growth or virulence on hop, indicating that VaaPV1 establishes a cryptic infection without detectable phenotypic effects under the conditions tested. Overall, our findings demonstrate that VaaPV1 is highly prevalent, genetically conserved and stably maintained in hop-associated V. nonalfalfae, providing a baseline for future studies of the ecological and biological significance of mycovirus infections in this vascular wilt pathogen.

• Jezik: Angleški jezik
• Ključne besede: hop, mycovirus, VaaPV1, Verticillium wilt
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2026
• Št. strani: 12 str.
• Številčenje: Vol. 75, Iss. 5, art. e70298
• PID: 20.500.12556/RUL-190604
• UDK: 578
• ISSN pri članku: 0032-0862
• URN: https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.70298
• DOI: 10.1111/ppa.70298
• COBISS.SI-ID: 303217411
• Datum objave v RUL: 30.10.2026

Gradivo je del revije

• Naslov: Plant Pathology
• Skrajšan naslov: Plant Pathol.
• Založnik: Her Majesty's Stationery Office
• ISSN: 0032-0862
• COBISS.SI-ID: 5661191

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: hmelj, mikovirusi, verticilijska uvelost

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: Z4-50137
• Naslov: Mikovirusi uporabni za biološko zatiranje fitopatogene glive Verticillium nonalfalfae

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0077
• Naslov: Kmetijske rastline - genetika in sodobne tehnologije