Vpliv mezenhimskih matičnih celic in njihovih zunajceličnih veziklov na proliferacijo in citokinski odziv celic roženičnega epitelija

Mravlje, Luka (avtor), Maličev, Elvira (mentor)

Namen magistrskega dela je bil ovrednotiti vpliv mezenhimskih matičnih celic (MSC) iz popkovine (UM), maščobnega tkiva (AT) in kostnega mozga (BM) ter njihovih zunajceličnih veziklov (MSC-EV) na celjenje in citokinski odziv humanih roženičnih epitelijskih celic (hCEC) in vitro. Učinek na celjenje smo spremljali s testom zapiranja epitelija z umetno povzročeno rano, pri katerem smo MSC uporabili v sistemu za kokultivacijo, MSC-EV pa v treh koncentracijah. Površino rane smo kvantificirali s programom ImageJ. Vnetni odziv smo sprožili z lipopolisaharidom (LPS), koncentracije izbranih mediatorjev pa določili z metodo Luminex xMAP. Po 4 urah so AT-MSC dosegle 52,90 ± 6,27 % zaprtja rane, BM-MSC 44,55 ± 11,95 %, UM-MSC 34,26 ± 11,72 %, negativna kontrola pa 36,81 ± 9,32 %. Statistično značilna razlika je bila ugotovljena med AT-MSC in UM-MSC (p = 0,0311). MSC-EV so prav tako spodbujali zapiranje rane, vendar učinek ni bil linearno odvisen od njihove koncentracije. Po 72 urah so bile pri več skupinah z MSC-EV koncentracije IP-10, IL-8, TNF-α, IL-1α in ICAM-1 nižje od kontrolnih vrednosti, medtem ko odziv drugih mediatorjev ni bil enoten. Rezultati kažejo, da MSC-EV ohranijo pomemben del regenerativnega in imunomodulatornega učinka starševskih MSC, vendar njihov učinek ostaja odvisen od vira, časa in eksperimentalnih pogojev.

• Jezik: Slovenski jezik
• Ključne besede: mezenhimske matične celice, zunajcelični vezikli, roženica, epitelij, celjenje rane, citokini
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2026
• PID: 20.500.12556/RUL-188429
• COBISS.SI-ID: 292544515
• Datum objave v RUL: 23.09.2026

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: The effect of mesenchymal stem cells and their extracellular vesicles on proliferation and cytokine response of corneal epithelial cells

Izvleček

The aim of this master’s thesis was to evaluate the effects of mesenchymal stem cells (MSCs) derived from umbilical cord (UM), adipose tissue (AT), and bone marrow (BM), and their extracellular vesicles (MSC-EVs), on wound healing and cytokine responses of human corneal epithelial cells (hCECs) in vitro. Wound healing was assessed using a scratch-wound assay, with MSCs applied in a transwell co-culture system and MSC-EVs tested at three concentrations. Wound area was quantified using ImageJ. Inflammation was induced with lipopolysaccharide (LPS), and selected inflammatory mediators were quantified using Luminex xMAP technology. After 4 h, wound closure reached 52.90 ± 6.27% with AT-MSCs, 44.55 ± 11.95% with BM-MSCs, 34.26 ± 11.72% with UM-MSCs, and 36.81 ± 9.32% in the negative control. A statistically significant difference was observed between AT-MSCs and UM-MSCs (p = 0.0311). MSC-EVs also promoted wound closure, although their effect was not linearly dose-dependent. After 72 h, several MSC-EV groups showed lower concentrations of IP-10, IL-8, TNF-α, IL-1α, and ICAM-1 than the control, whereas changes in other mediators were less consistent. These findings indicate that MSC-EVs retain an important part of the regenerative and immunomodulatory activity of their parental MSCs, although their effects depend on tissue source, time, and experimental conditions.

• Ključne besede: mesenchymal stem cells, extracellular vesicles, cornea, epithelium, wound healing, cytokines