The dataset contains a comprehensive phenotypic, physiological, and biochemical characterisation of tomato plants (Solanum lycopersicum L. cv. ‘Valido F1’) exposed to temperature stress under different biostimulant application timings. Plants were monitored during a 4-day stress period and a 9-day recovery phase across six timepoints. The dataset integrates high-throughput phenotyping, chlorophyll fluorescence imaging, gas exchange measurements, and targeted metabolite profiling, providing insights into plant stress responses, recovery dynamics, and the effects of the biostimulant Amalgerol® Essence under our specific research conditions.
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