Establishment of a low-level laser irradiation protocol for in vitro development of bovine embryos

Plavšić, Aleksandar (avtor), Zorc, Minja (avtor), Dovč, Peter (avtor)

Photobiomodulation using low-level laser irradiation has been proposed as a non-thermal approach for stimulating cellular metabolism, but practical protocols for its application to bovine embryos after fertilisation remain insufficiently described. This study aimed to establish a practical low-level laser irradiation procedure at different time points during the in vitro development of bovine embryos. Abattoir-derived oocytes were matured, fertilised with semen from a single bull, and cultured under standard conditions. Entire culture dishes were irradiated using a K-Laser Cube at 800 nm and 0.10 W for 200 s, delivering 20 J, on day one, three or five after fertilisation; an untreated run served as a reference. Embryos were evaluated on day seven according to International Embryo Transfer Society standards. Across four separate production runs, 1345 inseminated oocytes yielded 348 blastocysts meeting IETS stage 6, quality grade 1 criteria (26% overall); blastocyst rates were 27.8%, 22.0%, 25.9% and 28.5% for the reference, day one, day three and day five runs. Because each treatment was tested in a separate run, these values are descriptive and do not permit assessment of treatment efficacy. The procedure was incorporated into routine bovine in vitro embryo production and provides a basis for future validation studies.

• Jezik: Angleški jezik
• Ključne besede: cattle, in vitro embryo production, photobiomodulation, low-level laser, blastocyst rate, embryo transfer
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.03 - Drugi znanstveni članki
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2026
• Št. strani: 8 str.
• Številčenje: Vol. 13, issue 9, [article no.] 913
• PID: 20.500.12556/RUL-186917
• UDK: 636.2
• ISSN pri članku: 2306-7381
• DOI: 10.3390/vetsci13090913
• COBISS.SI-ID: 290164995
• Datum objave v RUL: 07.09.2026

Gradivo je del revije

• Naslov: Veterinary sciences
• Skrajšan naslov: Vet. sci.
• Založnik: MDPI AG
• ISSN: 2306-7381
• COBISS.SI-ID: 523429657

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: govedo, embriotransfer, razvoj zarodkov, lasersko obsevanje

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0220
• Naslov: Primerjalna genomika in genomska biodiverziteta