This thesis examined the effects of different hormone supplemented media on root system development in the model plant Nicotiana benthamiana, as well as antagonistic interactions among selected fungal strains. Plants were grown on media containing different concentrations of the plant growth regulators IBA, BAP and NAA, and on a control medium without added growth regulators. Root development, callus and shoot formation, and overall plant growth were monitored. The most developed root system was observed in plants grown on the control medium, while callus or shoot formation predominated on most hormone-supplemented media. In the second part of the study, interactions among six fungal strains from the genera Fusarium and Trichoderma were monitored on PDA medium. Antagonistic interactions were evaluated based on the formation of inhibition zones and the absence of colony overgrowth. Such interactions were observed only in some combinations, mainly between strains of the genus Trichoderma. The results show that the tested plant growth regulators did not improve root development compared with the control medium and that antagonistic interactions depended on the specific combination of fungal strains.
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