Vrednotenje vsebnosti resveratrola v trdnih disperzijah z mezoporoznimi nosilci

Brajkovič Mihelič, Nike (avtor), Zvonar Pobirk, Alenka (mentor)

Resveratrol je naravni polifenol z antioksidativnimi, protivnetnimi in drugimi potencialnimi terapevtskimi učinki, vendar njegovo uporabo omejujeta predvsem slaba vodotopnost in nizka biološka uporabnost. Eden izmed obetavnih pristopov za izboljšanje biofarmacevtskih lastnosti resveratrola je priprava amorfne trdne disperzije, pri kateri imajo pomembno vlogo izbira polimera, delež učinkovine ter uporaba mezoporoznih nosilcev. Namen diplomskega dela je bil ovrednotiti vsebnost resveratrola v amorfnih trdnih disperzijah, pripravljenih z metodo iztiskanja talin, ter ugotoviti vpliv deleža resveratrola, vrste polimera in vrste mezoporoznega nosilca na vsebnost resveratrola takoj po pripravi ter po trimesečnem pospešenem staranju. Analizirane formulacije so vsebovale 20, 30 ali 40 % (m/m) resveratrola, kot polimer sta bila uporabljena Eudragit® E PO in HPMC AS v kombinaciji s PEG 6000, kot mezoporozna nosilca pa Syloid® 244 FP in Neusilin® US2. Vsebnost resveratrola je bila določena z reverznofazno HPLC z detektorjem z nizom diod, rezultati pa so bili statistično analizirani z analizo variance. Rezultati so pokazali, da delež resveratrola v proučevanem območju ni statistično značilno vplival na vsebnost resveratrola niti takoj po pripravi niti po pospešenem staranju. Nasprotno je imel izbor polimera največji vpliv na ohranjanje vsebnosti resveratrola. Formulacije z zmesmi HPMC AS in PEG 6000 so po trimesečnem pospešenem staranju ohranile najvišjo vsebnost resveratrola, medtem ko so bile največje izgube opažene pri formulacijah s samim Eudragitom® E PO. Vrsta mezoporoznega nosilca je statistično značilno vplivala na spremembo vsebnosti med staranjem, pri čemer so formulacije z Neusilinom® US2 v povprečju bolje ohranile vsebnost resveratrola kot formulacije s Syloidom® 244 FP. Med proučevanimi dejavniki niso bile ugotovljene statistično značilne interakcije. Rezultati kažejo, da je za ohranjanje vsebnosti resveratrola v amorfnih trdnih disperzijah ključnega pomena predvsem ustrezna izbira polimera, medtem ko imata delež resveratrola in izbira mezoporoznega nosilca manj izrazit vpliv. Kot najprimernejše formulacije za nadaljnjo karakterizacijo so se izkazale formulacije z zmesmi HPMC AS in PEG 6000, saj so med proučevanimi sistemi izkazale najboljšo stabilnost med pospešenim staranjem.

• Jezik: Slovenski jezik
• Ključne besede: resveratrol, amorfne trdne disperzije, iztiskanje talin, mezoporozni nosilci, stabilnost
• Vrsta gradiva: Diplomsko delo/naloga
• Tipologija: 2.11 - Diplomsko delo
• Organizacija: FFA - Fakulteta za farmacijo
• Založnik: [N. Brajkovič Mihelič]
• Leto izida: 2026
• PID: 20.500.12556/RUL-186594
• UDK: 615.45.014:543(043.2)
• COBISS.SI-ID: 290342659
• Datum objave v RUL: 03.09.2026

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Characterization of resveratrol content in solid dispersions based on mesoporous carriers

Izvleček

Resveratrol is a natural polyphenol with antioxidant, anti-inflammatory, and other potential therapeutic properties. However, its pharmaceutical application is primarily limited by its poor aqueous solubility and low bioavailability. One of the most promising approaches to improve its biopharmaceutical properties is the preparation of amorphous solid dispersions, in which polymer selection, drug loading, and the use of mesoporous carriers play important roles. The aim of this thesis was to evaluate the resveratrol content in amorphous solid dispersions prepared by hot-melt extrusion and to investigate the influence of resveratrol loading, polymer type, and mesoporous carrier type on resveratrol content immediately after preparation and after three months of accelerated stability testing. The investigated formulations contained 20, 30, or 40% (w/w) resveratrol. Eudragit® E PO and HPMC AS in combination with PEG 6000 were used as polymeric carriers, while Syloid® 244 FP and Neusilin® US2 served as mesoporous carriers. Resveratrol content was determined by reversed-phase HPLC with a diode array detector, and the results were statistically evaluated using analysis of variance. The results demonstrated that resveratrol loading within the investigated range had no statistically significant effect on resveratrol content either immediately after preparation or after accelerated stability testing. In contrast, polymer selection had the greatest influence on preserving resveratrol content. Formulations containing HPMC AS and PEG 6000 retained the highest resveratrol content after three months of accelerated stability testing, whereas the greatest losses were observed in formulations containing only Eudragit® E PO. The type of mesoporous carrier also had a statistically significant effect on changes in resveratrol content during storage, with formulations containing Neusilin® US2 showing better preservation of resveratrol content than those containing Syloid® 244 FP. No statistically significant interactions were observed among the investigated factors. The results indicate that appropriate polymer selection is the key factor in preserving resveratrol content in amorphous solid dispersions, whereas resveratrol loading and the choice of mesoporous carrier have a less pronounced effect. Formulations containing HPMC AS and PEG 6000 proved to be the most suitable candidates for further characterization, as they exhibited the highest stability among the investigated systems during accelerated stability testing.

• Ključne besede: resveratrol, amorphous solid dispersions, hot-melt extrusion, mesoporous carriers, stability