The aim of this thesis was to investigate the patterns of lead (Pb) and iron (Fe) uptake and accumulation in three fungal species: oyster mushroom (Pleurotus ostreatus), lion’s mane mushroom (Hericium erinaceus), and deceiver (Laccaria laccata), and to evaluate their potential for use in mycoremediation. The fungi were cultivated on potato dextrose agar supplemented with Pb, Fe, or both metals at concentrations of 25, 100, and 200 mg/L. Cultivation lasted for 28, 35, and 43 days, and relative metal concentrations in the mycelium and substrate were determined using X-ray fluorescence spectrometry (XRF), after the cultivation. The results showed that the metal accumulation capacity differed among the studied species. In P. ostreatus, the concentration of Pb in the mycelium generally increased with increasing Pb concentration in the substrate, reaching 88 ppm after 43 days of cultivation. The highest Fe uptake was observed in L. laccata, where the Fe concentration increased from 109 ppm at 25 mg/L to 1202 ppm at 200 mg/L. In H. erinaceus, the most pronounced effect was observed under simultaneous exposure to Pb and Fe, as the concentrations of both metals in the mycelium increased at higher metal concentrations. The results confirm selective metal uptake and indicate that both the initial metal concentration and the interactions between metal ions influence metal accumulation in fungi. The studied fungal species represent promising organisms for further research in the field of mycoremediation.
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