Disturbed Antioxidant Capacity in Patients with systemic sclerosis associates with lung and gastrointestinal symptoms

Brezovec, Neža (avtor), Perdan-Pirkmajer, Katja (avtor), Burja, Blaž (avtor), Rotar, Žiga (avtor), Osredkar, Joško (avtor), Sodin-Šemrl, Snežna (avtor), Lakota, Katja (avtor), Čučnik, Saša (avtor)

The correct balance between reactive oxygen species and antioxidant defense in an organism is disturbed in oxidative stress. To assess oxidative balance in 36 SSc patients and 26 healthy controls (HCs), we measured reactive oxidative metabolites (ROMs), total antioxidant capacity (TAC), lipid peroxidation (measuring 4-HNE), and DNA oxidative damage (measuring 8-OHdG) in serum. Furthermore, DNA breaks in leukocytes of 35 SSc patients and 32 HCs were evaluated using COMET. While we report high ROMs for both SSc patients and age/sex matched HC samples, there was a significant increase in TAC in SSc patients as compared to HCs, and thus also a significantly higher oxidative stress index in SSc patients. TAC was significantly higher in SSc patients with ILD and gastrointestinal involvement, as well as in patients with anti-topoisomerase antibodies. We observe no difference in serum lipid peroxidation status or oxidative DNA damage. However, SSc patients had significantly more leukocyte DNA breaks than HCs; the most damage was observed in patients treated with immunosuppressives. Thus, our study confirms presence of oxidative stress and increased DNA damage in leukocytes of SSc patients; however, it points toward increased antioxidant capacity, which needs to be further studied.

• Jezik: Angleški jezik
• Ključne besede: systemic sclerosis, oxidative stress, reactive oxidative metabolites, antioxidants, lipid peroxidation, DNA damage, DNA breaks
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 16 str.
• Številčenje: Vol. 11, iss. 8, art. 2110
• PID: 20.500.12556/RUL-185503
• UDK: 616-002
• ISSN pri članku: 2227-9059
• DOI: 10.3390/biomedicines11082110
• COBISS.SI-ID: 159972611
• Datum objave v RUL: 07.08.2026

Gradivo je del revije

• Naslov: Biomedicines
• Skrajšan naslov: Biomedicines
• Založnik: MDPI AG
• ISSN: 2227-9059
• COBISS.SI-ID: 523006745

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: sistemska skleroza, oksidativni stres, reaktivni oksidativni metaboliti, antioksidanti, peroksidacija lipidov, poškodbe DNK, DNK zlomi

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: J7-3153
• Naslov: Molekularni mehanizmi specifičnosti pri uravnavanju izločanja in delovanja citokinov mišičnega izvora

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P3-0314
• Naslov: Sistemske avtoimunske bolezni