Bone growth and metabolism in broilers exposed to dietary PUFA or cyclical heat stress under two levels of dietary vitamins and minerals

Leskovec, Jakob (avtor), Levart, Alenka (avtor), Rezar, Vida (avtor), Pirman, Tatjana (avtor), Salobir, Janez (avtor), Vrecl, Milka (avtor), Brankovič, Jana (avtor)

Broilers are highly susceptible to heat stress (HS), which can impair bone growth and metabolism. Supplementation with n-3 polyunsaturated fatty acids (PUFA) may affect the metabolism of mineralized tissues, whereas antioxidants can mitigate these effects. The aim was to investigate the combined effects of HS (thermoneutral conditions vs. cyclic HS) or dietary oxidative stress induced by supranutritional n-3 PUFA (control feed without PUFA vs. feed supplemented with 5% linseed oil) with two levels (lower and higher) of dietary antioxidants and minerals on bone properties. The morphology and geometry of the proximal tibiotarsal growth plate, biomechanical behavior, and mineral composition of 42-day-old male Ross 308 broilers were evaluated. HS and high PUFA levels decreased bone size and mass, whereas supplementation with high antioxidant levels had the opposite effect. High PUFA supplementation induced histopathological changes in the growth plate that resembled tibial dyshondroplasia; these effects were attenuated by supplementation with high levels of antioxidants. Additionally, HS had a negative effect on the biomechanics and reduced the crude ash content. High levels of antioxidants increased the bone ash content, fat content, Ca content and Ca:P ratio. Our results suggest that HS and PUFA impair the growth of bones, hindering the overall growth of fast-growing broilers. The incidence of dyshondroplasia appears to be related to diet rather than to environmental changes. High antioxidant levels mitigate the negative effects of both stressors to some extent, suggesting that antioxidants have positive effects on bone growth and functionality, especially under nonoptimal environmental conditions and dietary stress.

• Jezik: Angleški jezik
• Ključne besede: antioxidant, bone growth and metabolism, broiler, heat stress, polyunsaturated fatty acid
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta ; VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2026
• Št. strani: 14 str.
• Številčenje: Vol. 17, art. 1771630
• PID: 20.500.12556/RUL-184449
• UDK: 636.52/.58:636.085:591.4:612.397
• ISSN pri članku: 1664-042X
• DOI: 10.3389/fphys.2026.1771630
• COBISS.SI-ID: 283867395
• Datum objave v RUL: 08.07.2026

Gradivo je del revije

• Naslov: Frontiers in physiology
• Skrajšan naslov: Front. physiol.
• Založnik: Frontiers Research Foundation
• ISSN: 1664-042X
• COBISS.SI-ID: 1218939

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: antioksidant, rast in metabolizem kosti, brojlerji, vročinski stres, večkrat nenasičena maščobna kislina

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0097-2019
• Naslov: Prehrana in mikrobna ekologija prebavil

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0053-2019
• Naslov: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih