Membrane palmitoylated protein 7 is required for osteogenesis and is linked with bone mineralisation and osteoporosis : the functional evaluation of GEFOS GWAS hit

Malavašič, Petra (avtor), Lojk, Jasna (avtor), Lovšin, Nika (avtor), Komadina, Radko (avtor), Haring, Gregor (avtor), Trebše, Rihard (avtor), Rivadeneira, Fernando (avtor), Karasik, David (avtor), Ostanek, Barbara (avtor), Marc, Janja (avtor)

Genome-wide association studies have identified multiple loci associated with bone mineral density, a major determinant of osteoporotic fracture risk. At one such locus, genetic, bioinformatic, and zebrafish knockout data strongly prioritize membrane palmitoylated protein 7 (MPP7) as a candidate gene, although its precise role in bone biology remains poorly defined. MPP7 encodes a member of the p55 Stardust family of membrane-associated guanylate kinase proteins, which are key regulators of epithelial cell polarity and junctional organization. Here, we investigated the functional role of MPP7 in bone biology. We found that MPP7 expression was significantly reduced—by approximately twofold—in bone tissue from osteoporotic patients compared with osteoarthritic patients and non-osteoporotic controls. Furthermore, we generated a CRISPR/Cas9-mediated MPP7 knockout in the human osteosarcoma HOS cell line and demonstrated that MPP7 deletion impairs osteogenic differentiation and completely abrogates mineralization through downregulation of ALPL expression. Knockout cells also displayed altered morphology, suggesting that MPP7 influences osteoblast function via effects on cell polarity and adhesion. Collectively, our findings, together with zebrafish genetic evidence, indicate that MPP7 plays a critical role in osteoblast differentiation and mineralization and may contribute to osteoporosis susceptibility in humans.

• Jezik: Angleški jezik
• Ključne besede: MPP7, adipogenesis, HOS cell lineage, human bone tissue, human muscle tissue
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 12 str.
• Številčenje: Vol. 116, iss. 1, art. 128
• PID: 20.500.12556/RUL-178945
• UDK: 61:340.6
• ISSN pri članku: 1432-0827
• DOI: 10.1007/s00223-025-01439-w
• COBISS.SI-ID: 254301443
• Datum objave v RUL: 02.02.2026

Gradivo je del revije

• Naslov: Calcified tissue international : and musculoskeletal research
• Skrajšan naslov: Calcif. tissue int.
• Založnik: Springer Nature
• ISSN: 1432-0827
• COBISS.SI-ID: 513641241

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: adipogeneza, celična linija HOS, človeško kostno tkivo, človeško mišično tkivo