Valorisation of acid whey permeate for high-purity nisin Z production using artisanal Lactococcus lactis isolates

Liaqat, Humna (avtor), Paveljšek, Diana (avtor), Oberčkal, Jernej (avtor), Bogovič Matijašić, Bojana (avtor)

The growing demand for sustainable bioprocessing has positioned whey, a side stream of the dairy industry, as a valuable substrate for producing high-value compounds. This study investigated the production and purification of nisin, a potent lantibiotic, using permeate (10 kDa cut-off ) of ultrafiltered acid whey (PUFAW) devoid of lactoferrin as growth medium. Three strains of Lactococcus lactis subsp. lactis, including two artisanal isolates (IM 143 and IM 145) and a reference strain (IM 407), were evaluated for nisin production in PUFAW supplemented with yeast extract (YE) and mineral salts. Strain IM 145 showed superior growth and antimicrobial activity in PUFAW with YE (30 g/L) and produced up to 51,200 BA/mL of nisin. The bacteriocin was purified by ammonium sulphate precipitation followed by preparative RP-HPLC, yielding a product with 79.2% chromatographic purity. Tricine-SDSPAGE and mass spectrometry confirmed the identity of the purified compound as nisin Z. This study presents a cost-effective, circular economy approach for the production of high-purity nisin from dairy side streams, with potential applications in food preservation and biomedicine.

• Jezik: Angleški jezik
• Ključne besede: nisin Z, bacteriocin, acid whey permeate, Lactococcus lactis, RP-HPLC, circular economy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 25.11.2025
• Leto izida: 2026
• Št. strani: 10 str.
• Številčenje: Vol. 26, article no. 9
• PID: 20.500.12556/RUL-177920
• UDK: 637.1
• ISSN pri članku: 1471-2180
• DOI: 10.1186/s12866-025-04543-x
• COBISS.SI-ID: 259442947
• Datum objave v RUL: 13.01.2026

Gradivo je del revije

• Naslov: BMC microbiology
• Skrajšan naslov: BMC Microbiol
• Založnik: BioMed Central
• ISSN: 1471-2180
• COBISS.SI-ID: 2441492

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mlekarstvo, kisla sirotka, nizin Z, Lactococcus lactis, krožno gospodarstvo

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0097
• Naslov: Prehrana in mikrobna ekologija prebavil

• Financer: Drugi - Drug financer ali več financerjev
• Naslov: LIFE for Acid Whey