Potential of the citrus bark cracking viroid (CBCVD) to infect hemp (Cannabis sativa L.)

Rostohar, Zala (avtor), Murovec, Jana (avtor), Volk, Helena (avtor)

Hemp (Cannabis sativa L.) faces increasing threats from various pathogens, including Cocadviroid latenshumuli also known as Hop Latent Viroid (HLVd), which causes dudding disease. Cocadviroid rimocitri, also known as Citrus Bark Cracking Viroid (CBCVd) severely affects hops, a close relative of hemp, raising concerns about its potential to infect hemp. This study investigated whether CBCVd can infect hemp and assessed tissue culture as a model for viroid research. Additionally, the effectiveness of in vitro synthesized viroid transcripts versus plant-derived RNA for infection was compared. Hemp tissue cultures were established and inoculated with CBCVd and HLVd transcripts or CBCVd and HLVd containing RNA. RT-PCR and sequencing confirmed infections, with transcript-based inoculation proving more effective than RNA-derived inoculation. CBCVd successfully infected hemp, expanding its known host range. No symptoms were observed in tissue cultures, but ex vitro acclimatized plants showed reduced growth, with HLVd-infected plant displaying the more severe effects than CBCVd-infected plant. This study provides the first evidence that CBCVd can artificially infect hemp under controlled conditions, highlighting potential transmission risks in agricultural settings. Additionally, tissue culture proved a valuable tool for viroid research, with transcript-based inoculation offering higher efficiency.

• Jezik: Angleški jezik
• Ključne besede: HLVd, CBCVd, dudding disease, in vitro transcription, Cocadviroid
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: Str. 267-274
• Številčenje: Volǂ 52, No. 2
• PID: 20.500.12556/RUL-175836
• UDK: 631
• ISSN pri članku: 0534-0012
• DOI: 10.2298/GENSR2502267R
• COBISS.SI-ID: 256581379
• Datum objave v RUL: 10.11.2025

Gradivo je del revije

• Naslov: Genetika : časopis Saveza društava genetičara Jugoslavije
• Skrajšan naslov: Genetika
• Založnik: Unija bioloških naučnih društava Jugoslavije
• ISSN: 0534-0012
• COBISS.SI-ID: 5143

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Srbski jezik (cirilica)
• Naslov: Potencijal viroida pucanja kore citrusa (CBCVD) da inficira konoplju (Cannabis sativa L.)
• Ključne besede: Cannabis sativa, konoplja, viroid razpokanosti skorje agrumov, okužba

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0077
• Naslov: Kmetijske rastline - genetika in sodobne tehnologije