Successful micropropagation of critically endangered Thymus bovei Benth : a wild medicinal plant strictly endemic to the Jordanian environment

Alkhawaldah, Refad Y. (avtor), Shibli, Rida A. (avtor), Tahtamouni, Reham W. (avtor), Shatnawi, Mohamad A. (avtor), Al-Qudah, Tamadour (avtor), Al-Qudah, Tamara S. (avtor)

Thymus bovei Benth. (Lamiaceae), locally referred to as Zateer Barry, is a critically endangered native plant species in Jordan (Taifour and EL-Oqlah, 2014), renowned for its exceptional medicinal properties. This study aims to establish a reliable micropropagation technique to prevent T. boveifrom extinction. The research focused on explant establishment, shoot multiplication, rooting, callus induction, and acclimatization of T. bovei. Explants were established in a half-strength Murashige and Skoog (MS) media supplemented with different concentrations of gibberellic acid (GA3). Successful shoot multiplication was achieved by subculturing nodal segments onto half-strength MS medium supplemented with kinetin (KIN), thidiazuron (TDZ), or benzylaminopurine (BA). Among the treatments, the most effective medium for shoot multiplication was supplemented with 2.5 mg l-1 KIN and 0.5 mg l-1 GA₃, which resulted in an average of 7.50 ± 0.54 micro-shoots per explant and a shoot length of 2.33 ± 0.18 cm. Rooting was most effective on half-strength MS media with 1.0 mg l-1 indole-3-butyric acid (IBA), yielding an average of 5.29 roots per microshoot. The highest callus development, measured by fresh mass (0.601 g), occurred in half-strength MS media with 2.0 mg l-1 2,4-D, while no callus formed with naphthaleneacetic acid (NAA). Acclimatized plants showed a 90 % survival rate when transferred to greenhouse conditions.

• Jezik: Angleški jezik
• Ključne besede: endangered plant, Jordan, medicinal plant, micropropagation, tissue culture, Thymus bovei
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 11 str.
• Številčenje: Vol. 121, no. 3
• PID: 20.500.12556/RUL-175182
• UDK: 63
• ISSN pri članku: 1854-1941
• DOI: 10.14720/aas.2025.121.3.18920
• COBISS.SI-ID: 253923843
• Datum objave v RUL: 20.10.2025

Gradivo je del revije

• Naslov: Acta agriculturae Slovenica
• Založnik: Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Biotehniška fakulteta, Oddelek za agronomijo in Oddelek za zootehniko, Univerza v Ljubljani, Biotehniška fakulteta, Založba Univerze v Ljubljani
• ISSN: 1854-1941
• COBISS.SI-ID: 219581184

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Naslov: Uspešna razmnožitev kritično ogrožene vrste materine dušice (Thymus bovei Benth), samonikle zdravilne rastline iz Jordanije
• Ključne besede: ogrožene rastline, Jordanija, zdravilne rastline, mikropropagacija, tkivne kulture, Thymus bovei