Bacterial and chemical interactions with iron oxide/hydroxide stalagmites using ATR-IR spectroscopy and its 4th derivative spectra

Kovač, Minka (avtor), Bele, Marjan (avtor), Orel, Boris (avtor), Mihelčič, Mohor (avtor)

Here we report on the chemical interaction between bacteria and fast-growing iron oxide/hydroxide sulphate stalagmites in the abandoned Sitarjevec mine in Slovenia with a low pH (2.2 to 3.4) environment. SEM micrographs revealed distinct morphologies of dry particles from both water and stalagmite layers, as well as the presence of bacterial cells, EPS residue, and biofilm. Bacterial adhesion was studied on the stalagmite surface and inside the stalagmite. Comparison of ATR-IR spectra and the corresponding 2nd and 4th derivative spectra revealed the presence of the P-OFe, COO▫$^⁻$▫ and SO▫$_4^{2-}$▫ modes, which are thought to be responsible for bacterial adhesion to the surface and were detected also in the inner part of the stalagmite. To our knowledge, this is the first study of its kind to confirm persistent chemical interactions between iron oxide/hydroxide sulphate-based stalagmite minerals and bacteria on their surface, which is also maintained in the core of the stalagmite.

• Jezik: Angleški jezik
• Ključne besede: bacterial adhesion, bacteria, geochemistry, infrared spectroscopy, mineralogy, X-ray spectroscopy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FS - Fakulteta za strojništvo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 15 str.
• Številčenje: Vol. 44
• PID: 20.500.12556/RUL-175130
• UDK: 550.4
• ISSN pri članku: 1616-1068
• DOI: 10.1007/s10230-025-01071-y
• COBISS.SI-ID: 246645763
• Datum objave v RUL: 17.10.2025

Gradivo je del revije

• Naslov: Mine water and the environment
• Skrajšan naslov: Mine water environ.
• Založnik: Springer
• ISSN: 1616-1068
• COBISS.SI-ID: 512028697

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: adhezija bakterij, bakterije, geokemija, infrardeča spektroskopija, mineralogija, rentgenska spektroskopija

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P2-0393
• Naslov: Napredni materiali za nizkoogljično in trajnostno družbo

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P2-0264
• Naslov: Trajnostni Polimerni materiali in Tehnologije