Analiza genetskih dejavnikov za količino maščob v mleku pri rjavi pasmi goveda : magistrsko delo

Kozjek, Alenka (avtor), Kunej, Tanja (mentor), Šimon, Martin (komentor)

Mlečna maščoba je ena ključnih lastnosti mleka, saj vpliva na njegovo prehransko in tehnološko vrednost. V magistrskem delu smo pri rjavi pasmi goveda preučili genetsko ozadje lastnosti količine mlečnih maščob z analizo 65 SNP-jev, ki so kazali najmočnejšo povezavo z lastnostjo na podlagi podatkov Interbull InterGenomics. Z uporabo zbirke Ensembl smo ugotovili, da je 40 SNP-jev intronskih, 20 je intergenskih, pet pa jih v zbirki Ensembl ni bilo evidentiranih. Največ SNP-jev je bilo lociranih na kromosomih 5, 11, 14 in 19, znotraj kandidatnih genov, kot so GHR, PLEC in MGST1. Podatke o genih, znotraj oziroma ob katerih se nahajajo SNP-ji, smo primerjali z zbirko QTLdb, kjer smo preverili prekrivanje z že znanimi kvantitativni lokusi za lastnosti mlečnosti. Ugotovili smo, da je bilo 16 genov predhodno že povezanih s količino mlečnih maščob. Predlagali smo tudi nove potencialne kandidate, kot so SHISA9, BORCS5 in PRKAG2. Nekateri geni, kot so TANC2, EGFLAM in GINS4, so bili poleg obravnavane lastnosti povezani še z drugimi lastnostmi mlečnosti. Analiza interakcij je razkrila funkcionalne povezave med nekaterimi geni. Rezultati prispevajo k boljšemu razumevanju kompleksne genetske regulacije sestave in prireje mleka ter predstavljajo osnovo za nadaljnje raziskave in uporabo v genomski selekciji pri rjavi pasmi goveda.

• Jezik: Slovenski jezik
• Ključne besede: govedoreja, pasme, rjava pasma, genetika, selekcija, mleko, lastnosti, maščobe, količina
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Kraj izida: Ljubljana
• Založnik: A. Kozjek
• Leto izida: 2025
• Št. strani: 1 spletni vir (1 datoteka PDF (IX, [48] str.))
• PID: 20.500.12556/RUL-174480
• UDK: 636.2:575(043.2)
• COBISS.SI-ID: 251607811
• Datum objave v RUL: 03.10.2025

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Analysis of genetic factors for milk fat yield in brown swiss cattle : M. Sc. thesis

Izvleček

Milk fat is one of the key traits in dairy production influencing both the nutritional and technological value. In this master’s thesis, we investigated the genetic background of the trait milk fat yield in Brown Swiss cattle by analyzing 65 single nucleotide polymorphisms (SNPs) showing the strongest association with the trait, based on data from the Interbull InterGenomics database. Using the Ensembl database, we found that 40 SNPs were intronic, 20 intergenic, and five were not recorded in Ensembl. Most SNPs were located on chromosomes 5, 11, 14, and 19, within candidate genes such as GHR, PLEC and MGST1. Information on the genes within or near which the SNPs were located was compared with the QTLdb database, where we verified overlap with previously reported quantitative trait loci (QTLs) for milk production traits. We confirmed that 16 genes have previously been associated with milk fat yield. We also proposed new potential candidate genes, such as SHISA9, BORCS5 and PRKAG2. Some genes, including TANC2, EGFLAM and GINS4, were associated not only with the studied trait but also with other milk production traits. The analysis of interactions revealed functional connections between certain genes. The results contribute to a better understanding of the complex genetic regulation of milk composition and yield and provide a foundation for further research and potential application in genomic selection in Brown Swiss cattle.

• Ključne besede: cattle breeding, breeds, Brown Swiss, genetics, selection, milk fat yield