Integrating selective precipitation of ▫$\alpha$▫-lactalbumin into a whey valorisation cascade

Prevc, Tjaša (avtor), Lokar, Blaž (avtor), Kete, Marko (avtor), Zabukovec, Polona (avtor), Bogovič Matijašić, Bojana (avtor), Zupančič Justin, Maja (avtor), Oberčkal, Jernej (avtor)

In the Balkans, and probably elsewhere, whey from small dairies, especially acid whey, still poses an environmental challenge. Despite recent advances in whey processing technology, whey is still largely treated as waste or ends up in biogas plants. Due to its high lactic acid content, acid whey cannot be concentrated by conventional drying or used directly in food. However, it contains several valuable components that can be converted into products with high added value. For this purpose, a valorisation cascade was developed, part of which is the extraction of lactoferrin and lactoperoxidase from acid whey or sweet whey. The remaining whey was concentrated and used for the acidic precipitation of α-lactalbumin. In small-scale laboratory experiments, a purity of α-lactalbumin of 72.9 % and 24.8 % was achieved, when isolated from acid whey and sweet whey, respectively. The isolation procedure was then demonstrated on a pilot scale, using 70 L of acid whey, which yielded 220 g of wet isolate of α-lactalbumin with a purity of 78.1 % and a high lysine (11.1 %) and leucine (10.0 %) content. The remaining whey fraction could be used in various fermentation processes or other applications.

• Jezik: Angleški jezik
• Ključne besede: mlekarstvo, stranski proizvodi, sirotka, laktalbumin
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 01.10.2025
• Leto izida: 2025
• Št. strani: Str. 231-239
• Številčenje: Vol. 75, no. 4
• PID: 20.500.12556/RUL-173786
• UDK: 637.35
• ISSN pri članku: 0026-704X
• DOI: 10.15567/mljekarstvo.2025.0403
• COBISS.SI-ID: 248068099
• Datum objave v RUL: 23.09.2025

Gradivo je del revije

• Naslov: Mljekarstvo : proizvodnja proučavanje i tehnologija mlijeka i mliječnih proizvoda
• Skrajšan naslov: Mljekarstvo
• Založnik: Hrvatsko mljekarsko društvo
• ISSN: 0026-704X
• COBISS.SI-ID: 85783

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Projekti

• Financer: Drugi - Drug financer ali več financerjev
• Številka projekta: C3330-18-952002
• Naslov: LAKTIKA - Frakcioniranje in oplemenitenje sirotkinih proteinov ter izraba preostanka za oblikovanje novih funkcionalnih živil in prehranskih dopolnil
• Akronim: ESRR