The thesis focuses on the ability of self-pollination or self-fertilization in two common buckwheat varieties, 'Trdinova' and 'Darja', in the absence of pollinators. The plants were grown in a greenhouse in five spatial isolations, according to the pin/thrum flower morphs. We analyzed 38 different genotypes, including 29 of 'Trdinova' variety and 9 of 'Darja' variety, as well as their F1 progeny. Seeds were obtained from 8, pin/thrum combinations of 'Trdinova' and 5 of 'Darja'. On average, plants with pin flowers produced 9.7 % more seeds than thrum plants. DNA was isolated from the leaves of both generations and used for genetic analyses with 8 microsatellite markers to determine similarities and differences between parents and progeny. Using agarose gel electrophoresis, the amplified DNA fragments were visualized and scored so that we could determine their presence and size. Capillary electrophoresis provided allele size data, which was then analyzed using GeneMapper 6 software. Genetic variability parameters were calculated using Cervus, while the graphical representation of genetic relationships was created using DARwin 6. In 'Trdinova' and its progeny, 37 alleles were amplified, with an average of 5 alleles per locus and an average polymorphism information content (PIC) of 0.421. In 'Darja' and its progeny, 34 alleles were amplified, with an average of 4 alleles per locus and a similar average PIC value of 0.415. In both varieties, which are pollinated by insects and show heterostyly, the degree of self-pollination is different and is on average 9.7 % higher in pin plants.
|
