Skeletal muscle death from the perspective of electrical impedance as evidenced by experiment and numerical modelling

Šmerc, Rok (avtor), Miklavčič, Damijan (avtor), Mahnič-Kalamiza, Samo (avtor)

Understanding the biophysical changes in skeletal muscle tissue during the minutes to hours post-excision or following irreversible damage is critical for biomedical applications and food processing. Muscle tissue, composed of myofibrillar and sarcoplasmic proteins, water, lipids, and connective tissue, forms a complex network of interactions that persists as it degrades post-mortem. This study investigates skeletal muscle death through ex vivo experimental measurements on porcine muscle, supported by a novel numerical model that builds the muscle up from individual fibres. Skeletal muscle tissue was found to exhibit strong anisotropy in electrical conductivity due to its structure. It demonstrates much lower conductivity perpendicular to muscle fibres compared to parallel with them owing to its limited plasma membrane conductivity. We explore how postmortem changes, including increased membrane permeability during membrane decomposition, and external interventions like electroporation, alter these anisotropic properties. Our findings have implications for biomedicine, specifically treatments targeting muscle tissue, such as pulsed field ablation for cardiac arrhythmias, and characterisation of in-vitro engineered muscle tissues. In food production, the study informs applications of pulsed electric fields to modify meat structure and texture. By integrating experimental and theoretical approaches, this work provides new insights into the electrochemical and structural dynamics of skeletal muscle during and after death.

• Jezik: Angleški jezik
• Ključne besede: tissue electrical impedance, ex vivo animal tissue, numerical modelling, skeletal muscle anisotropy, electroporation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 13 str.
• Številčenje: Vol. 197, part B, art. 111073
• PID: 20.500.12556/RUL-173338
• UDK: 602.621
• ISSN pri članku: 1879-0534
• DOI: 10.1016/j.compbiomed.2025.111073
• COBISS.SI-ID: 249062403
• Datum objave v RUL: 16.09.2025

Gradivo je del revije

• Naslov: Computers in biology and medicine
• Skrajšan naslov: Comput. biol. & med.
• Založnik: Elsevier
• ISSN: 1879-0534
• COBISS.SI-ID: 23205637

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: električna impedanca tkiva, živalsko tkivo ex vivo, numerično modeliranje, anizotropija skeletnih mišic, elektroporacija