Microcystis aeruginosa msoT1/msoA1 locus displays features of a type I toxin–antitoxin system

Ruparčič, Matija (avtor), Dolinar, Marko (avtor)

Type I toxin–antitoxin (TA) systems consist of a protein toxin that exerts a cytostatic or cytotoxic effect and an antisense RNA antitoxin that prevents translation of the toxin. Although well studied, type I TA systems have so far only been discovered in bacteria from the phyla Proteobacteria, Firmicutes, and Tenericutes. We hypothesized that type I systems could also be present in Cyanobacteria. Through bioinformatic analysis of the Microcystis aeruginosa PCC 7806SL genome, we discovered ten putative type I TA loci and characterized six of them experimentally. Two of the six putative type I toxins, BH695_0320 and MsoT1 (BH695_4017), were observed to negatively affect Escherichia coli cell growth, with MsoT1 exerting a phenotype similar to SrnB, a known type I toxin. We focused on the MsoT1/MsoA1 TA system and confirmed the expression of MsoT1 and MsoA1 in our assay. Additionally, we found that MsoA1 delays the toxic effects of MsoT1, indicating its role as a cognate antitoxin of MsoT1. Our results suggest that MsoT1/MsoA1 represents a novel candidate type I TA system, the first to be discovered in the Cyanobacteria phylum.

• Jezik: Angleški jezik
• Ključne besede: type I toxin-antitoxin systems, cyanobacteria, Microcystis aeruginosa, bacterial toxins, novel toxin-antitoxin systems
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 01.08.2025
• Leto izida: 2025
• Št. strani: 25 str.
• Številčenje: Vol. 17, iss. 8, art. 360
• PID: 20.500.12556/RUL-171107
• UDK: 577:582.232
• ISSN pri članku: 2072-6651
• DOI: 10.3390/toxins17080360
• COBISS.SI-ID: 243643907
• Datum objave v RUL: 05.08.2025

Gradivo je del revije

• Naslov: Toxins : Elektronski vir
• Skrajšan naslov: Toxins
• Založnik: MDPI
• ISSN: 2072-6651
• COBISS.SI-ID: 517594649

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: sistemi toksin-antitoksin tipa I, cianobakterije, Microcystis aeruginosa, bakterijski toksini, novi sistemi toksin-antitoksin

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P1-0048
• Naslov: Strukturna biologija