The aim of the bachelor's thesis was the design and implementation of temperature control for
a developmental bioreactor. A bioreactor is a key device in biotechnological processes that
enables the controlled cultivation of various bacterial strains. The bioreactor at CO BIK is
mainly used for cultivating the bacterium Escherichia coli (E. coli). E. coli is frequently used
as a host in bioprocesses for the production of plasmid DNA, which has wide applications in
biomedical and pharmaceutical fields. The process involves the optimization of bacterial
growth conditions, such as temperature, pH, dissolved oxygen concentration, and the
composition of the growth medium. To ensure the appropriate temperature, traditional smaller
bioreactors use a thermal jacket. I demonstrated that adequate temperature control is also
possible using a heating and cooling finger, which replaces the thermal jacket in the
developmental bioreactor. With PI temperature control using the heating and cooling finger, we
provide the conditions required by the biological process of bacterial cultivation. I first carried
out the temperature control procedure in the bioreactor without using bacteria. After the
successful PI control of the heating and cooling finger, the cultivation of bacteria was
successfully executed. During the writing of the thesis, I completed the project. The use of the
developmental bioreactor enables stable and reproducible production of plasmids, which is
crucial for further applications in synthetic biology, genetic engineering, and personalized
medicine. The developmental bioreactor is currently used for the development and optimization
of processes for the production of plasmid DNA (pDNA).
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