Vitamin A-enriched diet increases urothelial cell proliferation by upregulating Itga3 and Areg after cyclophosphamide-induced injury in mice

Dragar, Brina (avtor), Kranjc Brezar, Simona (avtor), Čemažar, Maja (avtor), Jesenko, Tanja (avtor), Romih, Rok (avtor), Erdani-Kreft, Mateja (avtor), Kuret, Tadeja (avtor), Zupančič, Daša (avtor)

Vitamin A (VitA) is an essential nutrient, affecting many cell functions, such as proliferation, apoptosis, and differentiation, all of which are important for the regeneration of various tissues. In this study, we investigated the effects of a VitA-enriched diet on the regeneration of the urothelium of the urinary bladder in mice after cyclophosphamide (CP)-induced injury. Female mice were fed VitA-enriched and normal diet for 1 week before receiving an intraperitoneal injection of CP (150 mg/kg). Urinary bladders were removed 1 and 3 days after CP. On Day 1, RNA sequencing showed that VitA upregulated two Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways: the cell cycle and the PI3K-Akt pathway. This was confirmed by qPCR, which showed significantly increased expression of the Itga3 and Areg genes. In addition, the effect of VitA on the proliferation of urothelial cells was analyzed by immunohistochemistry of Ki-67, which confirmed an increased proliferation rate. No significant effects of the VitA-enriched diet were observed on the expression of apoptosis-related genes and on differentiation-related markers of superficial urothelial cells. Our results suggest that a VitA-enriched diet improves early urothelial regeneration after CP-induced injury by promoting cell proliferation.

• Jezik: Angleški jezik
• Ključne besede: apoptosis, cyclophosphamide, differentiation, injury, proliferation, urinary bladder, urothelium, vitamin A
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 14 str.
• Številčenje: Vol. 69, iss. 9, art. e70045
• PID: 20.500.12556/RUL-169064
• UDK: 616-006:576
• ISSN pri članku: 1613-4133
• DOI: 10.1002/mnfr.70045
• COBISS.SI-ID: 232348419
• Datum objave v RUL: 09.05.2025

Gradivo je del revije

• Naslov: Molecular nutrition & food research
• Skrajšan naslov: Mol. nut. food res.
• Založnik: Wiley
• ISSN: 1613-4133
• COBISS.SI-ID: 512824345

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: apoptoza, ciklofosfamid, diferenciacija, poškodba, širjenje, sečni mehur, urotelija, vitamin A