Evaluation of the effects of estrogens on endometrial cancer cells of different grades

Pavlič, Renata (avtor), Pušić Novak, Maja (avtor), Lanišnik-Rižner, Tea (avtor)

Endometrial cancer (EC) is the most common gynecological malignancy in the Western world. The molecular basis and effects of various agents are frequently studied in model EC cell lines, but the most commonly used cell lines Ishikawa, HEC-1-A, RL95-2 and KLE have not been thoroughly and systematically investigated. We characterized EC cell lines of different grades by reassessing the expression of estrogen receptors ERα, ERβ, and GPER by qPCR and Western blot and investigated the effects of estrogens, estrone-sulfate, estrone and estradiol on their proliferation, migration, and clonogenicity. Estradiol promoted the proliferation of grade 1 Ishikawa EC cells and grade 2 RL95-2 cells. Estrone and estrone sulfate also stimulated the proliferation of Ishikawa, showed a tendency to increase the proliferation of HEC-1-A and RL95-2 cells, but decreased the proliferation of KLE. Estrogens had no effect on the migration and clonogenicity of these four EC cell lines, however, there was a trend toward a smaller colony area for cells incubated with higher estrogen concentrations. We have previously shown that in EC estradiol forms from inactive estrone sulfate via the sulfatase pathway. This study showed that estrogens significantly promote the proliferation of grade 1 Ishikawa EC cells, and grade 2 RL95-2 and decrease the proliferation of grade 3 KLE cells. These differences in proliferation were associated with ERα positivity of Ishikawa cells and GPER expression in other cells.

• Jezik: Angleški jezik
• Ključne besede: clonogenicity, endometrial cancer, estradiol, estrone, estrone-sulfate, migration, proliferation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 13 str.
• Številčenje: Vol. 251, art. 106762
• PID: 20.500.12556/RUL-168993
• UDK: 61:577.2
• ISSN pri članku: 1879-1220
• DOI: 10.1016/j.jsbmb.2025.106762
• COBISS.SI-ID: 234835459
• Datum objave v RUL: 06.05.2025

Gradivo je del revije

• Naslov: The journal of steroid biochemistry and molecular biology
• Skrajšan naslov: J. steroid biochem. mol. biol.
• Založnik: Elsevier
• ISSN: 1879-1220
• COBISS.SI-ID: 57401603

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: klonogenost, rak endometrija, estradiol, estron, estron-sulfat, migracije, širjenje