The mutational landscape and functional effects of noncoding ultraconserved elements in human cancers

Bayraktar, Recep (avtor), Tang, Yitao (avtor), Dragomir, Mihnea P. (avtor), Kunej, Tanja (avtor)

The mutational landscape of phylogenetically ultraconserved elements (UCEs), especially those in noncoding DNAs (ncUCEs), and their functional relevance in cancers remain poorly characterized. Here, we perform a systematic analysis of whole-genome and in-house targeted UCE sequencing datasets from more than 3000 patients with cancer of 13,736 UCEs and demonstrate that ncUCE somatic alterations are common. Using a multiplexed CRISPR knockout screen in colorectal cancer cells, we show that the loss of several altered ncUCEs significantly affects cell proliferation. In-depth functional studies in vitro and in vivo further reveal that specific ncUCEs can be enhancers of tumor suppressors (such as ARID1B) and silencers of oncogenic proteins (such as RPS13). Moreover, several miRNAs located in ncUCEs are recurrently mutated. Mutations in miR-142 locus can affect the Drosha-mediated processing of precursor miRNAs, resulting in the down-regulation of the mature transcript. These results provide systematic evidence that specific ncUCEs play diverse regulatory roles in cancer.

• Jezik: Angleški jezik
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 17 str.
• Številčenje: Vol. 11, iss. 8
• PID: 20.500.12556/RUL-168484
• UDK: 575:616
• ISSN pri članku: 2375-2548
• DOI: 10.1126/sciadv.ado2830
• COBISS.SI-ID: 226767107
• Datum objave v RUL: 15.04.2025

Gradivo je del revije

• Naslov: Science advances
• Založnik: American Association for the Advancement of Science
• ISSN: 2375-2548
• COBISS.SI-ID: 523392025

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: genetika, medicina, bolezni, rak, UCE