Beyond dimerization : harnessing tetrameric coiled-coils for nanostructure assembly

Vidmar, Sara (avtor), Šmidlehner, Tamara (avtor), Aupič, Jana (avtor), Strmšek, Žiga (avtor), Ljubetič, Ajasja (avtor), Xiao, Fei (avtor), Hu, Guang (avtor), Liu, Chuan (avtor), Beck, Florian (avtor), Erdmann, Philipp S. (avtor), Jerala, Roman (avtor)

Versatile DNA and polypeptide-based structures have been designed based on complementary modules. However, polypeptides can also form higher oligomeric states. We investigated the introduction of tetrameric modules as a substitute for coiled-coil dimerization units used in previous modular nanostructures. Tetramerizing helical bundles can run in parallel or antiparallel orientation, expanding the number of topological solutions for modular nanostructures. Furthermore, this strategy facilitates the construction of nanostructures from two identical polypeptide chains. Importantly, tetrameric modules substantially stabilized protein nanostructures against air–water interface denaturation, enabling the determination of the first cryo-electron microscopy three-dimensional structure of a coiled-coil-based nanostructure, confirming the designed agreement of the modules forming a tetrahedral cage.

• Jezik: Angleški jezik
• Ključne besede: coiled-coils, protein origami, cryo-electron, microscopy, protein design
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 8 str.
• Številčenje: Vol. 64, iss. 12, art. e202422075
• PID: 20.500.12556/RUL-167918
• UDK: 577
• ISSN pri članku: 1521-3773
• DOI: 10.1002/anie.202422075
• COBISS.SI-ID: 221895427
• Datum objave v RUL: 20.03.2025

Gradivo je del revije

• Naslov: Angewandte Chemie : international edition
• Skrajšan naslov: Angew. Chem.
• Založnik: Wiley
• ISSN: 1521-3773
• COBISS.SI-ID: 21810181

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: biokemija, DNK, polipeptidi, nanostrukture