Nisin-loaded gelatin microparticles for the enhanced bioactivity of bacterial nanocellulose

Hren, Maša (avtor), Trček, Janja (avtor), Šakanović, Aleksandra (avtor), Obradović, Hristina (avtor), Erdani-Kreft, Mateja (avtor), Hribernik, Silvo (avtor), Gorgieva, Selestina (avtor)

Bacterial nanocellulose (BnC) is of immense importance in medicine, although its lack of bioactivity present intriguing issue. We propose a method to modify BnC with gelatin and nisin biomolecules, and explore their synergistic effect on the antimicrobial activity. Gelatin microparticles (without/with nisin loading) with a size ~0.5 μm and ~1.3 μm were prepared by spray drying and stabilised by dehydrothermal treatment. Modified BnC-based membranes supported the formation of biologically relevant minerals and were non-cytotoxic to human gingival fibroblast cells (HGF). The presence of gelatin microparticles improved the viability of HGF by approximately 20 %, due to the effect of gelatin alone, independent of the addition of nisin. BnC coated with a nisin/gelatin solution reduces the viability of HGF by about 20 %, but this negative effect is not observed by nisin coated gelatin microparticles. The cell viability of BnC membranes was above 90 % in both porcine and human urothelial cells. The antimicrobial activity study confirmed an inhibitory effect of membranes modified with nisin-coated microparticles or a gelatin/nisin solution against Staphylococcus aureus at a non-cytotoxic nisin dose (150 μg/mL). The study demonstrates the structural effects of gelatin and gelatin/nisin mixtures on the bioactivity of BnC and provides a rationale for the modification procedure.

• Jezik: Angleški jezik
• Ključne besede: bacterial nanocellulose, gelatin, nisin, spray-drying, antimicrobial function, bioactivity
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 15 str.
• Številčenje: Vol. 305, pt. 1, art. 141203
• PID: 20.500.12556/RUL-167802
• UDK: 577:543.2/.9
• ISSN pri članku: 1879-0003
• DOI: 10.1016/j.ijbiomac.2025.141203
• COBISS.SI-ID: 228411907
• Datum objave v RUL: 12.03.2025

Gradivo je del revije

• Naslov: International journal of biological macromolecules
• Skrajšan naslov: Int. j. biol. macromol.
• Založnik: Elsevier
• ISSN: 1879-0003
• COBISS.SI-ID: 107163139

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: bakterijska nanoceluloza, želatina, nizin, sušenje z razprševanjem, protimikrobno delovanje, bioaktivnost