Advances in copper speciation, isotopic ratio measurement, bioimaging, and single-cell analysis : a critical review

Kozlica, Katarina (avtor), Milačič Ščančar, Radmila (avtor), Reberšek, Martina (avtor), Čemažar, Maja (avtor), Uršič Valentinuzzi, Katja (avtor), Ščančar, Janez (avtor)

Copper (Cu) is an essential trace element associated with various physiological processes in the human body. In humans, approximately 75–95% of Cu in plasma is tightly bound to ceruloplasmin (Cp). The remaining Cu (exchangeable Cu) is loosely bound to human serum albumin (HSA) and, to a lesser extent, to alpha-2-macroglobulin (α2M) and low molecular mass (LMM) amino acids. Neurodegenerative and neoplastic diseases may have a significant impact on Cu metabolism. In serum of Wilson's disease patients, the exchangeable Cu content is increased, while in patients with certain forms of cancer, elevated Cu-Cp levels are observed. Early diagnosis and treatment are crucial for the effective management of Cu-related disorders. This review critically evaluates analytical methods for Cu speciation, isotopic analysis, bioimaging, and single-cell analysis, which provide valuable information for the diagnosis and treatment of Cu-related disorders. Analytical challenges and future needs for the development of new methodological approaches are also addressed.

• Jezik: Angleški jezik
• Ključne besede: human samples, copper ceruloplasmin, copper human serum albumin, analytical methods, speciation, isotopic ratio measurement, bioimaging, single-cell analysis, review, copper
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 24 str.
• Številčenje: Vol. 184, art. 118152
• PID: 20.500.12556/RUL-166673
• UDK: 543
• ISSN pri članku: 1879-3142
• DOI: 10.1016/j.trac.2025.118152
• COBISS.SI-ID: 222568707
• Datum objave v RUL: 21.01.2025

Gradivo je del revije

• Naslov: TrAC trends in analytical chemistry
• Skrajšan naslov: TrAC, Trends anal. chem.
• Založnik: Elsevier
• ISSN: 1879-3142
• COBISS.SI-ID: 23039493

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: človeški vzorci, analitične metode, pregledni članki, plazemski baker, merjenje izotopskega razmerja, speciacija, baker, homeostaza, patologija