Cytopathological assessment is an accurate method for identifying immunophenotypic features and BRCA1/2 mutations of high-grade serous carcinoma from ascites

Miceska, Simona (avtor), Škof, Erik (avtor), Novaković, Srdjan (avtor), Stegel, Vida (avtor), Jeričević, Anja (avtor), Grčar-Kuzmanov, Biljana (avtor), Smrkolj, Špela (avtor), Cvjetičanin, Branko (avtor), Bebar, Sonja (avtor), Globočnik Kukovica, Marta (avtor), Kloboves-Prevodnik, Veronika (avtor)

Background: High‐grade serous carcinoma (HGSC) is the most common and aggressive type of ovarian cancer, and it is often associated with ascites at presentation. The objective of this study was to evaluate the accuracy of cytopathology to identify immunophenotypic features of HGSC and BRCA1/2 mutations from ascites. Methods: The study included 45 patients with histologically confirmed primary HGSC and malignant ascites. Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, and Ki‐67 was performed on cytospins and cytoblocks prepared from ascites. Next‐generation sequencing (NGS) was used to detect germline/somatic BRCA1/2 mutations in the ascites. Both ICC and NGS results were compared with immunohistochemistry (IHC) and NGS results from tissue blocks of primary tumor. Cronbach α and χ$^2$ statistics, respectively, were used. Results: ICC/IHC results for PAX8, WT1, P53, and P16 showed good reliability between cytospins, cytoblocks, and tissue blocks (α > 0.75), whereas poor reliability and significant differences were observed for Ki‐67 between ascites and tissue blocks (α < 0.26; p < .001 [Kruskal–Wallis]). For germline BRCA1/2 mutations, 100% concordance was confirmed, but only 14% concordance was confirmed for somatic mutations. Conclusions: These results demonstrate that cytopathology is an accurate method for identifying immunophenotypic features of HGSC and detecting germline BRCA1/2 mutations from ascites. However, further investigation is required for assessing the proliferation activity of HGSC in ascites and for detecting somatic BRCA1/2 mutations.

• Jezik: Angleški jezik
• Ključne besede: ascites, BRCA1/2 mutations, cytopathology, high-grade serous carcinoma, immunocytochemistry, immunohistochemistry, immunophenotype
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 188-197
• Številčenje: Vol. 131, iss. 3
• PID: 20.500.12556/RUL-166535
• UDK: 616-07
• ISSN pri članku: 1934-662X
• DOI: 10.1002/cncy.22664
• COBISS.SI-ID: 131704067
• Datum objave v RUL: 17.01.2025

Gradivo je del revije

• Naslov: Cancer cytopathology
• Skrajšan naslov: Cancer cytopathol.
• Založnik: Wiley, American Cancer Society
• ISSN: 1934-662X
• COBISS.SI-ID: 1070715

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ascites, mutacije BRCA 1/2, citopatologija, visokomaligni rak

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0289
• Naslov: Značilnosti malignih neoplazem, pomembnih za diagnozo ter napoved poteka bolezni in izida zdravljenja