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Changes in mitochondrial membrane potential in in vitro electroporation with nano- and microsecond pulses : Tamara Polajžer ... [et al.]
ID
Polajžer, Tamara
(
Avtor
),
ID
Peng, Wencheng
(
Avtor
),
ID
Yao, Chenguo
(
Avtor
),
ID
Miklavčič, Damijan
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,93 MB)
MD5: FEA29B23BAF6F922C6F74C462442A2DC
URL - Izvorni URL, za dostop obiščite
https://www.liebertpub.com/doi/full/10.1089/bioe.2024.0007
Galerija slik
Izvleček
With the introduction of nanosecond (ns) pulses, it was suggested that such pulses could be used to permeabilize intracellular membranes, including the mitochondrial membrane. The results presented thus far, however, are not conclusive. Interestingly, the effect of longer microsecond (▫$\mu$▫s) pulses on changes in mitochondria has never been investigated. We, therefore, investigated the changes in mitochondrial membrane permeability through changes in mitochondrial membrane potential (MMP) in CHO and H9c2 cells after electroporation with 4 ns, 200 ns, and 100 ▫$\mu$▫s pulses. In the range of reversible electroporation, the decrease in MMP generally depended on the cell line. In CHO, ns pulses decreased MMP at lower electroporation intensities than μs. In H9c2, ns and ▫$\mu$▫s were equally effective. In the range of irreversible electroporation, MMP decreased even further, regardless of pulse duration and cell type. The analysis at different time points showed that the changes in MMP within the first hour after pulse treatment are dynamic. Our results on the efficacy of ns pulses are consistent with published data, but with this study we show that ▫$\mu$▫s pulses cause similar changes in MMP as ns pulses, demonstrating that electroporation affects MMP regardless of pulse duration. At the same time, however, differences in MMP changes were observed between different cell lines, indicating some dependence of MMP changes on cell type.
Jezik:
Angleški jezik
Ključne besede:
electroporation
,
nanosecond pulses
,
microsecond pulses
,
mitochondria
,
mitochondrial membranepotential
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FE - Fakulteta za elektrotehniko
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Založnik:
Mary Ann Liebert
Leto izida:
2024
Št. strani:
Str. 97-107
Številčenje:
Vol. 6, iss. 2
PID:
20.500.12556/RUL-160227
UDK:
602.621
ISSN pri članku:
2576-3113
DOI:
10.1089/bioe.2024.0007
COBISS.SI-ID:
200294659
Datum objave v RUL:
23.08.2024
Število ogledov:
36
Število prenosov:
3
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Objavi na:
Gradivo je del revije
Naslov:
Bioelectricity
Skrajšan naslov:
Bioelectricity
Založnik:
Mary Ann Liebert
ISSN:
2576-3113
COBISS.SI-ID:
114503171
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
elektroporacija
,
nanosekundni pulzi
,
mikrosekundni pulzi
,
mitohondriji
,
mitohondrijski membranski potencial
Projekti
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
P2-0249
Naslov:
Elektroporacija v biologiji, biotehnologiji in medicini
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Medtronic
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
China Scholarship Council
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
I0-0022
Naslov:
Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)
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