Changes in mitochondrial membrane potential in in vitro electroporation with nano- and microsecond pulses : Tamara Polajžer ... [et al.]

Polajžer, Tamara (author), Peng, Wencheng (author), Yao, Chenguo (author), Miklavčič, Damijan (author)

With the introduction of nanosecond (ns) pulses, it was suggested that such pulses could be used to permeabilize intracellular membranes, including the mitochondrial membrane. The results presented thus far, however, are not conclusive. Interestingly, the effect of longer microsecond (▫$\mu$▫s) pulses on changes in mitochondria has never been investigated. We, therefore, investigated the changes in mitochondrial membrane permeability through changes in mitochondrial membrane potential (MMP) in CHO and H9c2 cells after electroporation with 4 ns, 200 ns, and 100 ▫$\mu$▫s pulses. In the range of reversible electroporation, the decrease in MMP generally depended on the cell line. In CHO, ns pulses decreased MMP at lower electroporation intensities than μs. In H9c2, ns and ▫$\mu$▫s were equally effective. In the range of irreversible electroporation, MMP decreased even further, regardless of pulse duration and cell type. The analysis at different time points showed that the changes in MMP within the first hour after pulse treatment are dynamic. Our results on the efficacy of ns pulses are consistent with published data, but with this study we show that ▫$\mu$▫s pulses cause similar changes in MMP as ns pulses, demonstrating that electroporation affects MMP regardless of pulse duration. At the same time, however, differences in MMP changes were observed between different cell lines, indicating some dependence of MMP changes on cell type.

• Language: English
• Keywords: electroporation, nanosecond pulses, microsecond pulses, mitochondria, mitochondrial membranepotential
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Publisher: Mary Ann Liebert
• Year: 2024
• Number of pages: Str. 97-107
• Numbering: Vol. 6, iss. 2
• PID: 20.500.12556/RUL-160227
• UDC: 602.621
• ISSN on article: 2576-3113
• DOI: 10.1089/bioe.2024.0007
• COBISS.SI-ID: 200294659
• Publication date in RUL: 23.08.2024

Record is a part of a journal

• Title: Bioelectricity
• Shortened title: Bioelectricity
• Publisher: Mary Ann Liebert
• ISSN: 2576-3113
• COBISS.SI-ID: 114503171

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: elektroporacija, nanosekundni pulzi, mikrosekundni pulzi, mitohondriji, mitohondrijski membranski potencial