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AMPK and glucose deprivation exert an isoform-specific effect on the expression of Na$^+$,K$^+$-ATPase subunits in cultured myotubes
ID
Vidović, Anja
(
Avtor
),
ID
Dolinar, Klemen
(
Avtor
),
ID
Chibalin, Alexander V.
(
Avtor
),
ID
Pirkmajer, Sergej
(
Avtor
)
PDF - Predstavitvena datoteka,
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MD5: ABA33DCC2D054CB338FC0FE5F0B8DDDF
URL - Izvorni URL, za dostop obiščite
https://link.springer.com/article/10.1007/s10974-024-09673-9
Galerija slik
Izvleček
In skeletal muscle, Na$^+$,K$^+$-ATPase (NKA), a heterodimeric (α/β) P-type ATPase, has an essential role in maintenance of Na$^+$ and K$^+$ homeostasis, excitability, and contractility. AMP-activated protein kinase (AMPK), an energy sensor, increases the membrane abundance and activity of NKA in L6 myotubes, but its potential role in regulation of NKA content in skeletal muscle, which determines maximum capacity for Na$^+$ and K$^+$ transport, has not been clearly delineated. We examined whether energy stress and/or AMPK affect expression of NKA subunits in rat L6 and primary human myotubes. Energy stress, induced by glucose deprivation, increased protein content of NKAα1 and NKAα2 in L6 myotubes, while decreasing the content of NKAα1 in human myotubes. Pharmacological AMPK activators (AICAR, A-769662, and diflunisal) modulated expression of NKA subunits, but their effects only partially mimicked those that occurred in response to glucose deprivation, indicating that AMPK does not mediate all effects of energy stress on NKA expression. Gene silencing of AMPKα1/α2 increased protein levels of NKAα1 in L6 myotubes and NKAα1 mRNA levels in human myotubes, while decreasing NKAα2 protein levels in L6 myotubes. Collectively, our results suggest a role for energy stress and AMPK in modulation of NKA expression in skeletal muscle. However, their modulatory effects were not conserved between L6 myotubes and primary human myotubes, which suggests that coupling between energy stress, AMPK, and regulation of NKA expression in vitro depends on skeletal muscle cell model.
Jezik:
Angleški jezik
Ključne besede:
AMPK
,
energy stress
,
glucose
,
myotubes
,
Na$^+$
,
K$^+$-ATPase
,
skeletal muscle
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2024
Št. strani:
Str. 139-154
Številčenje:
Vol. 45, iss. 3
PID:
20.500.12556/RUL-160108
UDK:
616-092
ISSN pri članku:
1573-2657
DOI:
10.1007/s10974-024-09673-9
COBISS.SI-ID:
194665731
Datum objave v RUL:
21.08.2024
Število ogledov:
102
Število prenosov:
23
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Objavi na:
Gradivo je del revije
Naslov:
Journal of muscle research and cell motility
Skrajšan naslov:
J. muscle res. cell motil.
Založnik:
Springer Nature
ISSN:
1573-2657
COBISS.SI-ID:
513197337
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
energijski stres
,
glukoza
,
miocevke
,
skeletna mišica
Projekti
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
P3-0043
Naslov:
Molekularni mehanizmi razvoja in delovanja skeletne mišice
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
J7-3153
Naslov:
Molekularni mehanizmi specifičnosti pri uravnavanju izločanja in delovanja citokinov mišičnega izvora
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Novo Nordisk Research Foundation
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Karolinska Institutet, Strategic Research Programme in Diabetes
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Russian Scientific Foundation (RNF)
Številka projekta:
19-15-00118
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