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Evaluation of THP-1 and Jurkat cell lines coculture for the in vitro assessment of the effects of immunosuppressive substances
ID
Franko, Nina
(
Author
),
ID
Sollner Dolenc, Marija
(
Author
)
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MD5: EDE47FAF4EC0F92E303E6C95263D98CE
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https://www.mdpi.com/2305-6304/12/8/607
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Abstract
The strong appeal to reduce animal testing calls for the development and validation of in vitro, in chemico and in silico models that would replace the need for in vivo testing and ex vivo materials. A category that requires such new approach methods is the assessment of immunosuppression that can be induced by chemicals including environmental pollutants. To assess the immunosuppressive action on monocytes and lymphocytes, we mimicked the whole-blood cytokine-release assay by preparing an in vitro coculture of THP-1 and Jurkat cell lines. We optimised its activation and investigated the effects of known immunosuppressive drugs with different mechanisms of action on the release of proinflammatory cytokines. Decreased secretion of IL-8 was achieved by several immunosuppressive mechanisms and was therefore selected as an appropriate marker of immunosuppression. A set of environmentally occurring bisphenols, BPA, BPAP, BPP, BPZ, BPE, TCBPA and BPS-MAE, were then applied to the model and BPP and BPZ were found to act as potent immunosuppressants at micromolar concentrations.
Language:
English
Keywords:
immunosuppression
,
coculture
,
in vitro
,
new approach method
,
cell lines
,
bisphenols
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FFA - Faculty of Pharmacy
Publication status:
Published
Publication version:
Version of Record
Year:
2024
Number of pages:
13 str.
Numbering:
Vol. 12, iss. 8, art. 607
PID:
20.500.12556/RUL-160097
UDC:
615.37:612.43:620.266.1
ISSN on article:
2305-6304
DOI:
10.3390/toxics12080607
COBISS.SI-ID:
204624131
Publication date in RUL:
20.08.2024
Views:
104
Downloads:
37
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Record is a part of a journal
Title:
Toxics : Elektronski vir
Shortened title:
Toxics
Publisher:
MDPI
ISSN:
2305-6304
COBISS.SI-ID:
520262681
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
imunosupresija
,
kokultura
,
in vitro
,
metoda novega pristopa
,
celične linije
,
bisfenoli
,
imunosupresivna biološka zdravila
,
hormonski motilci
Projects
Funder:
EC - European Commission
Project number:
101057014
Name:
Partnership for the Assessment of Risks from Chemicals
Acronym:
PARC
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P1-0208
Name:
Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin
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