Evaluation of THP-1 and Jurkat cell lines coculture for the in vitro assessment of the effects of immunosuppressive substances

Franko, Nina (author), Sollner Dolenc, Marija (author)

The strong appeal to reduce animal testing calls for the development and validation of in vitro, in chemico and in silico models that would replace the need for in vivo testing and ex vivo materials. A category that requires such new approach methods is the assessment of immunosuppression that can be induced by chemicals including environmental pollutants. To assess the immunosuppressive action on monocytes and lymphocytes, we mimicked the whole-blood cytokine-release assay by preparing an in vitro coculture of THP-1 and Jurkat cell lines. We optimised its activation and investigated the effects of known immunosuppressive drugs with different mechanisms of action on the release of proinflammatory cytokines. Decreased secretion of IL-8 was achieved by several immunosuppressive mechanisms and was therefore selected as an appropriate marker of immunosuppression. A set of environmentally occurring bisphenols, BPA, BPAP, BPP, BPZ, BPE, TCBPA and BPS-MAE, were then applied to the model and BPP and BPZ were found to act as potent immunosuppressants at micromolar concentrations.

• Language: English
• Keywords: immunosuppression, coculture, in vitro, new approach method, cell lines, bisphenols
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 13 str.
• Numbering: Vol. 12, iss. 8, art. 607
• PID: 20.500.12556/RUL-160097
• UDC: 615.37:612.43:620.266.1
• ISSN on article: 2305-6304
• DOI: 10.3390/toxics12080607
• COBISS.SI-ID: 204624131
• Publication date in RUL: 20.08.2024

Record is a part of a journal

• Title: Toxics : Elektronski vir
• Shortened title: Toxics
• Publisher: MDPI
• ISSN: 2305-6304
• COBISS.SI-ID: 520262681

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: imunosupresija, kokultura, in vitro, metoda novega pristopa, celične linije, bisfenoli, imunosupresivna biološka zdravila, hormonski motilci

Projects

• Funder: EC - European Commission
• Project number: 101057014
• Name: Partnership for the Assessment of Risks from Chemicals
• Acronym: PARC

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P1-0208
• Name: Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin