Evaluation of THP-1 and Jurkat cell lines coculture for the in vitro assessment of the effects of immunosuppressive substances

Franko, Nina (avtor), Sollner Dolenc, Marija (avtor)

The strong appeal to reduce animal testing calls for the development and validation of in vitro, in chemico and in silico models that would replace the need for in vivo testing and ex vivo materials. A category that requires such new approach methods is the assessment of immunosuppression that can be induced by chemicals including environmental pollutants. To assess the immunosuppressive action on monocytes and lymphocytes, we mimicked the whole-blood cytokine-release assay by preparing an in vitro coculture of THP-1 and Jurkat cell lines. We optimised its activation and investigated the effects of known immunosuppressive drugs with different mechanisms of action on the release of proinflammatory cytokines. Decreased secretion of IL-8 was achieved by several immunosuppressive mechanisms and was therefore selected as an appropriate marker of immunosuppression. A set of environmentally occurring bisphenols, BPA, BPAP, BPP, BPZ, BPE, TCBPA and BPS-MAE, were then applied to the model and BPP and BPZ were found to act as potent immunosuppressants at micromolar concentrations.

• Jezik: Angleški jezik
• Ključne besede: immunosuppression, coculture, in vitro, new approach method, cell lines, bisphenols
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 13 str.
• Številčenje: Vol. 12, iss. 8, art. 607
• PID: 20.500.12556/RUL-160097
• UDK: 615.37:612.43:620.266.1
• ISSN pri članku: 2305-6304
• DOI: 10.3390/toxics12080607
• COBISS.SI-ID: 204624131
• Datum objave v RUL: 20.08.2024

Gradivo je del revije

• Naslov: Toxics : Elektronski vir
• Skrajšan naslov: Toxics
• Založnik: MDPI
• ISSN: 2305-6304
• COBISS.SI-ID: 520262681

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: imunosupresija, kokultura, in vitro, metoda novega pristopa, celične linije, bisfenoli, imunosupresivna biološka zdravila, hormonski motilci

Projekti

• Financer: EC - European Commission
• Številka projekta: 101057014
• Naslov: Partnership for the Assessment of Risks from Chemicals
• Akronim: PARC

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P1-0208
• Naslov: Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin