DNA preservation in compact and trabecular bone

Zupanič-Pajnič, Irena (avtor), Kovačič, Nika (avtor)

Significant variation exists in the molecular structure of compact and trabecular bone. In compact bone full dissolution of the bone powder is required to efficiently release the DNA from hydroxyapatite. In trabecular bone where soft tissues are preserved, we assume that full dissolution of the bone powder is not required to release the DNA from collagen. To investigate this issue, research was performed on 45 Second World War diaphysis (compact bone)-epiphysis (trabecular bone) femur pairs, each processed with a full dissolution (FD) and partial dissolution (PD) extraction method. DNA quality and quantity were assessed using qPCR PowerQuant analyses, and autosomal STRs were typed to confirm the authenticity of isolated DNA. Our results support different mechanisms of DNA preservation in compact and trabecular bone because FD method was more efficient than PD method only in compact bone, and no difference in DNA yield was observed in trabecular bone, showing no need for full dissolution of the bone powder when trabecular bone tissue is processed. In addition, a significant difference in DNA yield was observed between compact and trabecular bone when PD was applied, with more DNA extracted from trabecular bone than compact bone. High suitability of trabecular bone processed with PD method is also supported by the similar quantities of DNA isolated by FD method when applied to both compact and trabecular bone. Additionally similar quantities of DNA were isolated when compact bone was extracted with FD method and trabecular bone was extracted with PD method. Processing trabecular bone with PD method in routine identification of skeletonized human remains shortens the extraction procedure and simplifies the grinding process.

• Jezik: Angleški jezik
• Ključne besede: aged DNA, compact bone, DNA preservation, human identification, trabecular bone
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 6 str.
• Številčenje: Vol. 71, art. 103067
• PID: 20.500.12556/RUL-158969
• UDK: 61:575
• ISSN pri članku: 1878-0326
• DOI: 10.1016/j.fsigen.2024.103067
• COBISS.SI-ID: 199642627
• Datum objave v RUL: 24.06.2024

Gradivo je del revije

• Naslov: Forensic science international. Genetics
• Založnik: Elsevier, International Society for Forensic Genetics
• ISSN: 1878-0326
• COBISS.SI-ID: 32934361

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: stara DNA, kompaktna kost, ohranjanje DNA, identifikacija človeka, trabekularna kost

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-3080
• Naslov: Določitev predniškega porekla iz DNA za identifikacijske namene