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Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice
ID Umek, Nejc (Avtor), ID Pušnik, Luka (Avtor), ID Ugwoke, Chiedozie Kenneth (Avtor), ID Šink, Žiga (Avtor), ID Horvat, Simon (Avtor), ID Janáček, Jiří (Avtor), ID Cvetko, Erika (Avtor)

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Izvleček
It is not well-understood how type 1 diabetes (T1DM) affects skeletal muscle histological phenotype, particularly capillarisation. This study aimed to analyze skeletal muscle myosin heavy chain (MyHC) fibre type changes and 3D capillary network characteristics in experimental T1DM mice. Female C57BL/6J-OlaHsd mice were categorized into streptozotocin (STZ)-induced diabetic (n = 12) and age-matched non-diabetic controls (n =12). The muscle fibre phenotype of the soleus, gluteus maximus, and gastrocnemius muscles were characterized based on the expression of MyHC isoforms, while capillaries of the gluteus maximus were assessed with immunofluorescence staining, confocal laser microscopy and 3D image analysis. STZ-induced diabetic mice exhibited elevated glucose levels, reduced body weight, and prolonged thermal latency, verifying the T1DM phenotype. In both T1DM and non-diabetic mice, the gluteus maximus and gastrocnemius muscles predominantly expressed fast-twitch type 2b fibers, with no significant differences noted. However, the soleus muscle in non-diabetic mice had a greater proportion of type 2a fibers and comparable type 1 fiber densities (26.2 ± 14.6% vs 21.9 ± 13.5%) relative to diabetic mice. T1DM mice showed reduced fiber diameters (P = 0.026), and the 3D capillary network analysis indicated a higher capillary length per muscle volume in the gluteus maximus of diabetic mice compared to controls (P < 0.05). Overall, T1DM induced significant changes in the skeletal muscle, including shifts in MyHC fibre types, decreased fibre diameters, and increased relative capillarisation, possibly due to muscle fibre atrophy. Our findings emphasize the superior detail provided by the 3D analytical method for characterizing skeletal muscle capillary architecture, highlighting caution in interpreting 2D data for capillary changes in T1DM.

Jezik:Angleški jezik
Ključne besede:type 1 diabetes mellitus (T1DM), skeletal muscle, myosin heavy chain isoforms, capillary network analysis, muscle fibre composition, 3D image analysis
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2024
Št. strani:Str. 582-592
Številčenje:Vol. 24, iss. 3
PID:20.500.12556/RUL-158802 Povezava se odpre v novem oknu
UDK:611
ISSN pri članku:2831-0896
DOI:10.17305/bb.2023.9843 Povezava se odpre v novem oknu
COBISS.SI-ID:171111427 Povezava se odpre v novem oknu
Datum objave v RUL:20.06.2024
Število ogledov:277
Število prenosov:45
Metapodatki:XML DC-XML DC-RDF
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Gradivo je del revije

Naslov:Biomolecules & biomedicine
Založnik:Association of Basic Medical Sciences of FBIH
ISSN:2831-0896
COBISS.SI-ID:139613955 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:sladkorna bolezen tipa 1 (T1DM), skeletna mišica, izoforme težke verige miozina, analiza kapilarnih mrež, sestava mišičnih vlaken, analiza 3D slike

Projekti

Financer:ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:P3-0043
Naslov:Molekularni mehanizmi razvoja in delovanja skeletne mišice

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Ministry of Education, Youth and Sports of the Czech Republic
Številka projekta:LM2023050
Naslov:Large RI Project LM2023050 Czech-BioImaging

Financer:Drugi - Drug financer ali več financerjev
Program financ.:European Regional Development Fund
Številka projekta:CZ.02.1.01/0.0/0.0/18_046/0016045

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