Modelling bi-specific antibodies in aqueous solution

Hvozd, Taras (avtor), Kalyuzhnyi, Yurij V. (avtor), Vlachy, Vojko (avtor)

This study presents theoretical results for physico-chemical properties of system of molecules modeling bi-specific antibodies, such as, dual-variable-domain monoclonal antibodies (DVD-Ig) and Fabs-In-Tandem Immunoglobulin (FIT-Ig). These molecules are representatives of the engineered proteins that combine the function and specificity of two monoclonal antibodies. Individual molecules are here depicted as an assembly of nine (or in case of the Fit-Ig eleven) hard spheres, organized to resemble the Y-shaped object. The effects of the increased size, asymmetry, and flexibility of individual molecules on measurable properties of such systems of molecules are investigated. We examined the liquid-liquid phase separation, the second virial coefficient $B_2$, and viscosity under various experimental conditions. The calculations are compared with the data for regular monoclonal antibodies and discussed in view of the experimental results for DVD-Ig solutions available in literature.

• Jezik: Angleški jezik
• Ključne besede: physical chemistry, aqueous solutions, modelling, antibodies
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 7 str.
• Številčenje: Vol. 402, art. 124740
• PID: 20.500.12556/RUL-156204
• ISSN: 0167-7322
• UDK: 544
• DOI: 10.1016/j.molliq.2024.124740
• Datum objave v RUL: 14.05.2024

Gradivo je del revije

• Naslov: Journal of molecular liquids
• Skrajšan naslov: J. mol. liq.
• Založnik: Elsevier
• ISSN: 0167-7322
• COBISS.SI-ID: 23074821

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 08.05.2024

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: fizikalna kemija, vodne raztopine, modeliranje, antitelesca