Experimental and theoretical model for the origin of coiling of cellular protrusions around fibers

Kumar Sadhu, Raj (avtor), Hernandez-Padilla, Christian (avtor), Eshed Eisenbach, Yael (avtor), Penič, Samo (avtor), Zhang, Lixia (avtor), Vishwasrao, Harshad D. (avtor), Behkam, Bahareh (avtor), Konstantopoulos, Konstantinos (avtor), Shroff, Hari (avtor), Iglič, Aleš (avtor), Peles, Elior (avtor), Nain, Amrinder S. (avtor), Gov, Nir S. (avtor)

Protrusions at the leading-edge of a cell play an important role in sensing the extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around the extracellular fibers. However, the physics of this coiling process, and the mechanisms that drive it, are not well understood. We present a combined theoretical and experimental study of the coiling of cellular protrusions on fibers of different geometry. Our theoretical model describes membrane protrusions that are produced by curved membrane proteins that recruit the protrusive forces of actin polymerization, and identifies the role of bending and adhesion energies in orienting the leading-edges of the protrusions along the azimuthal (coiling) direction. Our model predicts that the cell’s leading-edge coils on fibers with circular cross-section (above some critical radius), but the coiling ceases for flattened fibers of highly elliptical cross-section. These predictions are verified by 3D visualization and quantitation of coiling on suspended fibers using DualView light-sheet microscopy (diSPIM). Overall, we provide a theoretical framework, supported by experiments, which explains the physical origin of the coiling phenomenon.

• Jezik: Angleški jezik
• Ključne besede: cell motility, computer simulations, Monte Carlo method, physical protein model
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 1-13
• Številčenje: Vol. 14, [article no.] 5612
• PID: 20.500.12556/RUL-155480
• UDK: 577
• ISSN pri članku: 2041-1723
• DOI: 10.1038/s41467-023-41273-y
• COBISS.SI-ID: 166115587
• Datum objave v RUL: 04.04.2024

Gradivo je del revije

• Naslov: Nature communications
• Skrajšan naslov: Nat. Commun.
• Založnik: Nature Publishing Group
• ISSN: 2041-1723
• COBISS.SI-ID: 2315876

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mobilnost celice, računalniške simulacije, metoda Monte Carlo, fizikalni model proteinov