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Integrative transcriptomic analysis in human and mouse model of anaphylaxis identifies gene signatures associated with cell movement, migration and neuroinflammatory signalling
ID
Rijavec, Matija
(
Avtor
),
ID
Maver, Aleš
(
Avtor
),
ID
Turner, Paul J.
(
Avtor
),
ID
Hočevar, Keli
(
Avtor
),
ID
Košnik, Mitja
(
Avtor
),
ID
Yamani, Amnah
(
Avtor
),
ID
Hogan, Simon P.
(
Avtor
),
ID
Custovic, Adnan
(
Avtor
),
ID
Peterlin, Borut
(
Avtor
),
ID
Korošec, Peter
(
Avtor
)
PDF - Predstavitvena datoteka,
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MD5: 09745DA4023A99BA96CA0AF25260B0D4
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.1016165/full
Galerija slik
Izvleček
Background: Anaphylaxis is an acute life-threatening allergic reaction and a concern at a global level; therefore, further progress in understanding the underlying mechanisms and more effective strategies for diagnosis, prevention and management are needed. Objective: We sought to identify the global architecture of blood transcriptomic features of anaphylaxis by integrating expression data from human patients and mouse model of anaphylaxis. Methods: Bulk RNA-sequencings of peripheral whole blood were performed in: i) 14 emergency department (ED) patients with acute anaphylaxis, predominantly to Hymenoptera venom, ii) 11 patients with peanut allergy undergoing double-blind, placebo-controlled food challenge (DBPCFC) to peanut, iii) murine model of IgE-mediated anaphylaxis. Integrative characterisation of differential gene expression, immune cell-type-specific gene expression profiles, and functional and pathway analysis was undertaken. Results: 1023 genes were commonly and significantly dysregulated during anaphylaxis in ED and DBPCFC patients; of those genes, 29 were also dysregulated in the mouse model. Cell-type-specific gene expression profiles showed a rapid downregulation of blood basophil and upregulation of neutrophil signature in ED and DBPCFC patients and the mouse model, but no consistent and/or significant differences were found for other blood cells. Functional and pathway analysis demonstrated that human and mouse blood transcriptomic signatures of anaphylaxis follow trajectories of upregulation of cell movement, migration and neuroinflammatory signalling, and downregulation of lipid activating nuclear receptors signalling. Conclusion: Our study highlights the matched and extensive blood transcriptomic changes and suggests the involvement of discrete cellular components and upregulation of migration and neuroinflammatory pathways during anaphylaxis.
Jezik:
Angleški jezik
Ključne besede:
transcriptome analysis
,
cell movement
,
migration
,
neuroinflammatory signaling
,
lipid activating nuclear receptors signaling
,
anaphylaxis
,
diagnosis
,
mast cells
,
basophils
,
neutrophils
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
MF - Medicinska fakulteta
FFA - Fakulteta za farmacijo
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2022
Št. strani:
15 str.
Številčenje:
Vol. 13, art. 1016165
PID:
20.500.12556/RUL-155258
UDK:
575
ISSN pri članku:
1664-3224
DOI:
10.3389/fimmu.2022.1016165
COBISS.SI-ID:
133240067
Datum objave v RUL:
22.03.2024
Število ogledov:
394
Število prenosov:
58
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in immunology
Skrajšan naslov:
Front. immunol.
Založnik:
Frontiers Media
ISSN:
1664-3224
COBISS.SI-ID:
30774233
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
gibanje celic
,
migracija
,
anafilaksija
,
diagnostika
,
mastociti
,
bazofilci
,
nevtrofilci
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P3-0360
Naslov:
Celostna obravnava alergijskih bolezni in astme v Sloveniji: od epidemiologije do genetike
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
NHS Trust, Imperial College Healthcare, NIHR, Biomedical Research Centre
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Imperial College London
Financer:
NIH - National Institutes of Health
Številka projekta:
AI138177
Financer:
NIH - National Institutes of Health
Številka projekta:
AI112626
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