Characterization of nanohybridosomes from lipids and spruce homogenate containing extracellular vesicles

Spasovski, Vesna (avtor), Romolo, Anna (avtor), Zagorc, Urška (avtor), Arrigler, Vesna (avtor), Kisovec, Matic (avtor), Bedina Zavec, Apolonija (avtor), Arko, Matevž (avtor), Molnár, Adrienn (avtor), Schlosser, Gitta (avtor), Iglič, Aleš (avtor), Kogej, Ksenija (avtor), Kralj-Iglič, Veronika (avtor)

Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized hybridosomes or nanohybridosomes, NSHs) were considered. Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy, interferometric light microscopy and liquid chromatography–mass spectrometry. Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the particles R$_h$ (between 140 and 180 nm), while the concentrations of the particles were in the range between 10$^{13}$ and 10$^{14}$/mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and proteins were found in hybridosomes. Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that large-scale production of NSHs from lipids and spruce needle homogenate is feasible.

• Jezik: Angleški jezik
• Ključne besede: hybridosomes, liposomes, nanovesicles, extracellular particles, small cellular particles, drug delivery
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta ; FE - Fakulteta za elektrotehniko; FKKT - Fakulteta za kemijo in kemijsko tehnologijo ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 1709-1721
• Številčenje: Vol. 19
• PID: 20.500.12556/RUL-154747
• UDK: 577:574.5
• ISSN pri članku: 1178-2013
• DOI: 10.2147/IJN.S432836
• COBISS.SI-ID: 186884099
• Datum objave v RUL: 28.02.2024

Gradivo je del revije

• Naslov: International journal of nanomedicine
• Založnik: Taylor & Francis, Dove Medical Press
• ISSN: 1178-2013
• COBISS.SI-ID: 3223828

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: hibridosomi, liposomi, nanovezikli, izvencelični vezikli, mali celični delci, prenos učinkovin