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The effect of phytosulfokine alpha on haploid embryogenesis and gene expression of Brassica napus microspore cultures
ID
Mestinšek Mubi, Špela
(
Avtor
),
ID
Kunej, Urban
(
Avtor
),
ID
Vogrinčič, Valentin
(
Avtor
),
ID
Jakše, Jernej
(
Avtor
),
ID
Murovec, Jana
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(5,27 MB)
MD5: 6DF577A90BF88DFAB12EB5B8F3F53AF8
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/articles/10.3389/fpls.2024.1336519
Galerija slik
Izvleček
Microspore embryogenesis (ME) is the most powerful tool for creating homozygous lines in plant breeding and molecular biology research. It is still based mainly on the reprogramming of microspores by temperature, osmotic and/or nutrient stress. New compounds are being sought that could increase the efficiency of microspore embryogenesis or even induce the formation of haploid embryos from recalcitrant genotypes. Among these, the mitogenic factor phytosulfokine alpha (PSK-α) is promising due to its broad spectrum of activity in vivo and in vitro. The aim of our study was to investigate the effect of PSK-α on haploid embryogenesis from microspores of oilseed rape (Brassica napus L., DH4079), one of the most important oil crops and a model plant for studying the molecular mechanisms controlling embryo formation. We tested different concentrations (0, 0.01, 0.1 and 1 µM) of the peptide and evaluated its effect on microspore viability and embryo regeneration after four weeks of culture. Our results showed a positive correlation between addition of PSK-α and cultured microspore viability and a positive effect also on the number of developed embryos. The analysis of transcriptomes across three time points (day 0, 2 and 4) with or without PSK-α supplementation (15 RNA libraries in total) unveiled differentially expressed genes pivotal in cell division, microspore embryogenesis, and subsequent regeneration. PCA grouped transcriptomes by RNA sampling time, with the first two principal components explaining 56.8% variability. On day 2 with PSK, 45 genes (15 up- and 30 down-regulated) were differentially expressed when PSK-α was added and their number increased to 304 by day 4 (30 up- and 274 down-regulated). PSK, PSKR, and PSI gene expression analysis revealed dynamic patterns, with PSK2 displaying the highest increase and overall expression during microspore culture at days 2 and 4. Despite some variations, only PSK1 showed significant differential expression upon PSK-α addition. Of 16 ME-related molecular markers, 3 and 15 exhibited significant differential expression in PSK-supplemented cultures at days 2 and 4, respectively. Embryo-specific markers predominantly expressed after 4 days of culture, with higher expression in medium without PSK, while on day 0, numerous sporophyte-specific markers were highly expressed.
Jezik:
Angleški jezik
Ključne besede:
PSK-α
,
PSK
,
haploids
,
doubled haploids
,
transcriptome
,
RNA-seq
,
mitogenic factor
,
differentially expressed genes
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2024
Št. strani:
13 str.
Številčenje:
Vol. 15, art. 1336519
PID:
20.500.12556/RUL-154506
UDK:
577.2
ISSN pri članku:
1664-462X
DOI:
10.3389/fpls.2024.1336519
COBISS.SI-ID:
183606531
Datum objave v RUL:
19.02.2024
Število ogledov:
262
Število prenosov:
23
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in plant science
Skrajšan naslov:
Front. plant sci.
Založnik:
Frontiers Research Foundation
ISSN:
1664-462X
COBISS.SI-ID:
3011663
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
žlahtnjenje rastlin
,
embriogeneza
,
haploidi
,
oljna ogrščica
,
tkivne kulture
,
kultura mikrospor
Projekti
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
P4-0077
Naslov:
Kmetijske rastline - genetika in sodobne tehnologije
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
J4-9307
Naslov:
Preurejanje genomov izbranih vrst rodu Brassica s tehnologijo CRISPR/Cas9
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
ARRS-MR-496
Naslov:
Mladi raziskovalci - ARRS
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
IO-0022-0481-001
Naslov:
Infrastructural centre
Akronim:
IC RRC-AG
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