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A simplified nasopharyngeal swab collection procedure for minimizing patient discomfort while retaining sample quality
ID
Uršič, Tina
(
Avtor
),
ID
Kogoj, Rok
(
Avtor
),
ID
Šikonja, Jaka
(
Avtor
),
ID
Jevšnik Virant, Monika
(
Avtor
),
ID
Petrovec, Miroslav
(
Avtor
)
PDF - Predstavitvena datoteka,
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(371,42 KB)
MD5: 850AB2CC977DC926F01F34B22AF6EA74
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/articles/10.3389/fpubh.2023.1066934/full
Galerija slik
Izvleček
A nasopharyngeal swab (NPS) is the most frequently collected sample type when molecular diagnosis of respiratory viruses, including SARS CoV-2, is required. An optimal collection technique would provide sufficient sample quality for the diagnostic process and would minimize the discomfort felt by the patient. This study compares a simplified NPS collection procedure with only one rotation of the swab to a more standard procedure with five rotations. Swabs were collected from 76 healthy volunteers by the same healthcare professional on 2 consecutive days at a similar hour to minimize variability. The number of Ubiquitin C copy number per sample was measured by real-time quantitative PCR and patient discomfort was assessed by questionnaire. No statistically significant difference (p = 0.15) was observed in the Ubiquitin C copy number per sample between a NPS collected with one rotation (5.2 ± 0.6 log UBC number copies/sample) or five rotations (5.3 ± 0.5 log UBC number copies/sample). However, a statistically significant difference was observed in discomfort between these two procedures, the second being much more uncomfortable. Additional analysis of the results showed a weak correlation between discomfort and the number of human cells recovered (Spearman's rho = 0.202) and greater discomfort in younger people. The results of this study show that a NPS collected with one slow rotation has the same quality as a NPS collected with five rotations. However, the collection time is shorter and, most importantly, less unpleasant for patients.
Jezik:
Angleški jezik
Ključne besede:
nasopharyngeal swab
,
collection procedure
,
sample quality
,
discomfort score
,
Ubiquitin C
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2023
Št. strani:
4 str.
Številčenje:
Vol. 11, art. 1066934
PID:
20.500.12556/RUL-154018
UDK:
616-07
ISSN pri članku:
2296-2565
DOI:
10.3389/fpubh.2023.1066934
COBISS.SI-ID:
146264835
Datum objave v RUL:
19.01.2024
Število ogledov:
403
Število prenosov:
27
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in public health
Skrajšan naslov:
Front. public health
Založnik:
Frontiers Media
ISSN:
2296-2565
COBISS.SI-ID:
523096857
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
nazofaringealni bris
,
postopek odvzema
,
kakovost vzorca
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P3-0083
Naslov:
Odnosi parazitskega obstajanja
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