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Alterations in immunophenotype and metabolic profile of mononuclear cells during follow up in children with multisystem inflammatory syndrome (MIS-C)
ID
Kopitar, Andreja Nataša
(
Avtor
),
ID
Repas, Jernej
(
Avtor
),
ID
Janžič, Larisa
(
Avtor
),
ID
Bizjak, Maša
(
Avtor
),
ID
Tajnšek Vesel, Tina
(
Avtor
),
ID
Emeršič, Nina
(
Avtor
),
ID
Zajc Avramovič, Mojca
(
Avtor
),
ID
Ihan, Alojz
(
Avtor
),
ID
Avčin, Tadej
(
Avtor
),
ID
Pavlin, Mojca
(
Avtor
)
PDF - Predstavitvena datoteka,
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MD5: A8F9B752647A6100BE6B4CDA110E3163
URL - Izvorni URL, za dostop obiščite
https://www.frontiersin.org/articles/10.3389/fimmu.2023.1157702/full
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Izvleček
Introduction: Although children seem to be less susceptible to COVID-19, some of them develop a rare but serious hyperinflammatory condition called multisystem inflammatory syndrome in children (MIS-C). While several studies describe the clinical conditions of acute MIS-C, the status of convalescent patients in the months after acute MIS-C is still unclear, especially the question of persistence of changes in the specific subpopulations of immune cells in the convalescent phase of the disease. Methods: We therefore analyzed peripheral blood of 14 children with MIS-C at the onset of the disease (acute phase) and 2 to 6 months after disease onset (post-acute convalescent phase) for lymphocyte subsets and antigen-presenting cell (APC) phenotype. The results were compared with six healthy age-matched controls. Results: All major lymphocyte populations (B cells, CD4 + and CD8+ T cells, and NK cells) were decreased in the acute phase and normalized in the convalescent phase. T cell activation was increased in the acute phase, followed by an increased proportion of γ/δ-double-negative T cells (γ/δ DN Ts) in the convalescent phase. B cell differentiation was impaired in the acute phase with a decreased proportion of CD21 expressing, activated/memory, and class-switched memory B cells, which normalized in the convalescent phase. The proportion of plasmacytoid dendritic cells, conventional type 2 dendritic cells, and classical monocytes were decreased, while the proportion of conventional type 1 dendritic cells was increased in the acute phase. Importantly the population of plasmacytoid dendritic cells remained decreased in the convalescent phase, while other APC populations normalized. Immunometabolic analysis of peripheral blood mononuclear cells (PBMCs) in the convalescent MIS-C showed comparable mitochondrial respiration and glycolysis rates to healthy controls. Conclusions: While both immunophenotyping and immunometabolic analyzes showed that immune cells in the convalescent MIS-C phase normalized in many parameters, we found lower percentage of plasmablasts, lower expression of T cell co-receptors (CD3, CD4, and CD8), an increased percentage of γ/δ DN Ts and increased metabolic activity of CD3/CD28-stimulated T cells. Overall, the results suggest that inflammation persists for months after the onset of MIS-C, with significant alterations in some immune system parameters, which may also impair immune defense against viral infections.
Jezik:
Angleški jezik
Ključne besede:
MIS-C
,
biomarkers
,
metabolism
,
B lymphocytes
,
T lymphocytes
,
monocytes
,
lymphocyte activation
,
dendritic cells
,
T lymphocytes exhaustion
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
FE - Fakulteta za elektrotehniko
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2023
Št. strani:
16 str.
Številčenje:
Vol. 14, art. 1157702
PID:
20.500.12556/RUL-153992
UDK:
616-097:616-053.2
ISSN pri članku:
1664-3224
DOI:
10.3389/fimmu.2023.1157702
COBISS.SI-ID:
151228931
Datum objave v RUL:
18.01.2024
Število ogledov:
713
Število prenosov:
36
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in immunology
Skrajšan naslov:
Front. immunol.
Založnik:
Frontiers Media
ISSN:
1664-3224
COBISS.SI-ID:
30774233
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
MIS-C
,
biomarkerji
,
metabolizem
,
B limfociti
,
T limfociti
,
monociti
,
aktivacija limfocitov
,
dendritske celice
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ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
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Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J3-3061
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Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
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Naslov:
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Financer:
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Program financ.:
University Medical Centre Ljubljana
Številka projekta:
20210069
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
University Medical Centre Ljubljana
Številka projekta:
20220090
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