CRISPR/Cas-mediated plant genome editing : outstanding challenges a decade after implementation

Cardi, Teodoro (author), Murovec, Jana (author), Bakhsh, Allah (author), Galović, Vladislava (author), Lukan, Tjaša (author), Yıldırım, Kubilay (author), Zlatković, Milica (author), Van Laere, Katrijn (author)

The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.

• Language: English
• Keywords: CRISPR applications, CRISPR platforms, gene regulations, mutant detection, plant regeneration
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 1144-1165
• Numbering: Vol. 28, iss. 10
• PID: 20.500.12556/RUL-153181
• UDC: 60
• ISSN on article: 1360-1385
• DOI: 10.1016/j.tplants.2023.05.012
• COBISS.SI-ID: 156416259
• Publication date in RUL: 20.12.2023

Record is a part of a journal

• Title: Trends in Plant Science
• Shortened title: Trends plant sci.
• Publisher: Elsevier Science
• ISSN: 1360-1385
• COBISS.SI-ID: 3735079

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: rastlinska genetika, aplikacije CRISPR, regulacija genov, mutanti, regeneracija rastlin

Projects

• Funder: Other - Other funder or multiple funders
• Funding programme: European Cooperation in Science and Technology
• Project number: CA18111
• Name: COST Action PlantEd