20.500.12556/RUL-153181 CRISPR/Cas-mediated plant genome editing outstanding challenges a decade after implementation The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants. CRISPR applications CRISPR platforms gene regulations mutant detection plant regeneration rastlinska genetika aplikacije CRISPR regulacija genov mutanti regeneracija rastlin true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-20 10:58:10 2023-12-20 10:58:12 2023-12-21 03:57:36 0000-00-00 00:00:00 2023 0 0 Str. 1144-1165 iss. 10 Vol. 28 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 60 1360-1385 10.1016/j.tplants.2023.05.012 156416259 CRISPR_Cas-mediated_plant_genome_editing__outstanding_challenges_a_decade_after_implementation.pdf CRISPR_Cas-mediated_plant_genome_editing__outstanding_challenges_a_decade_after_implementation.pdf 1 0774E4BAB59B9B522B933BC96DDCA108 32212d2761fb0974a07e205668ace8a9c164e682ec3608ce367612c1780a625b 9c03f467-9f1e-11ee-8a8e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179101 https://www.cell.com/action/showPdf?pii=S1360-1385%2823%2900164-4 1 4a5f092d-9f1e-11ee-8a8e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179100 Biotehniška fakulteta 0 0 0