CRISPR/Cas-mediated plant genome editing : outstanding challenges a decade after implementation

Cardi, Teodoro (avtor), Murovec, Jana (avtor), Bakhsh, Allah (avtor), Galović, Vladislava (avtor), Lukan, Tjaša (avtor), Yıldırım, Kubilay (avtor), Zlatković, Milica (avtor), Van Laere, Katrijn (avtor)

The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.

• Jezik: Angleški jezik
• Ključne besede: CRISPR applications, CRISPR platforms, gene regulations, mutant detection, plant regeneration
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 1144-1165
• Številčenje: Vol. 28, iss. 10
• PID: 20.500.12556/RUL-153181
• UDK: 60
• ISSN pri članku: 1360-1385
• DOI: 10.1016/j.tplants.2023.05.012
• COBISS.SI-ID: 156416259
• Datum objave v RUL: 20.12.2023

Gradivo je del revije

• Naslov: Trends in Plant Science
• Skrajšan naslov: Trends plant sci.
• Založnik: Elsevier Science
• ISSN: 1360-1385
• COBISS.SI-ID: 3735079

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: rastlinska genetika, aplikacije CRISPR, regulacija genov, mutanti, regeneracija rastlin

Projekti

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: European Cooperation in Science and Technology
• Številka projekta: CA18111
• Naslov: COST Action PlantEd