Vaš brskalnik ne omogoča JavaScript!
JavaScript je nujen za pravilno delovanje teh spletnih strani. Omogočite JavaScript ali pa uporabite sodobnejši brskalnik.
Nacionalni portal odprte znanosti
Odprta znanost
DiKUL
slv
|
eng
Iskanje
Brskanje
Novo v RUL
Kaj je RUL
V številkah
Pomoč
Prijava
Simultaneous determination of free biliverdin and free bilirubin in serum : a comprehensive LC-MS approach
ID
Albreht, Alen
(
Avtor
),
ID
Martelanc, Mitja
(
Avtor
),
ID
Žiberna, Lovro
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,74 MB)
MD5: F3510607A74C213BAF631BD37945E141
URL - Izvorni URL, za dostop obiščite
https://www.sciencedirect.com/science/article/pii/S0003267023012941
Galerija slik
Izvleček
Background: Prognosis, diagnosis, and treatment of several diseases strongly rely on the sensitive, selective, and accurate determination of specific biomarkers in relevant biological samples. Free biliverdin and free bilirubin represent important new biomarkers of oxidative stress, however, the lack of suitable analytical methods for their determination has hindered progress in biomedical and clinical research. Results: Here, we introduce a first comprehensive approach for robust and simultaneous determination of these bilins in serum using liquid chromatography – mass spectrometry (LC-MS). The developed analytical method exhibits linearity for both analytes within the concentration range of 0.5–100 nM, with limits of detection and quantitation determined at 0.1 nM and 0.5 nM, respectively. Moreover, several analytical pitfalls related to the intrinsic molecular structures of free bilirubin and free biliverdin and their trace concentration levels in biological samples are discussed here in detail for the first time. We have shown that the solubility, chemical stability, and affinity of these bilins to various materials strongly depend on the solvent, pH, and addition of stabilizing and chelating agents. Finally, the validated LC-MS method was successfully applied to the analysis of both bilins in fetus bovine serums, yielding higher free bilirubin/biliverdin ratios compared with previously reported values for human serum. Significance: Failure to recognize and address the challenges presented here often leads to substantial analytical errors and consequently biased interpretation of the obtained results. This pertains not only to LC-MS, but also to many other analytical platforms due to the compound-derived sources of error.
Jezik:
Angleški jezik
Ključne besede:
biomedical research
,
sensitivity
,
robustness
,
serum
,
stability
,
matrix effects
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
FFA - Fakulteta za farmacijo
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2024
Št. strani:
8 str.
Številčenje:
Vol. 1287, art. 342073
PID:
20.500.12556/RUL-152925
UDK:
543
ISSN pri članku:
1873-4324
DOI:
10.1016/j.aca.2023.342073
COBISS.SI-ID:
176790787
Datum objave v RUL:
12.12.2023
Število ogledov:
661
Število prenosov:
31
Metapodatki:
Citiraj gradivo
Navadno besedilo
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Kopiraj citat
Objavi na:
Gradivo je del revije
Naslov:
Analytica chimica acta
Založnik:
Elsevier
ISSN:
1873-4324
COBISS.SI-ID:
23064325
Licence
Licenca:
CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:
Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
analizna kemija
,
bolezni
,
zdravljenje
,
biomarkerji
,
serum
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P1-0005
Naslov:
Funkcionalna živila in prehranska dopolnila
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P3-0067
Naslov:
Farmakologija in farmakogenomika
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J3-8209
Naslov:
Bilirubin kot zaščitni dejavnik pred razvojem kroničnih degenerativnih bolezni: serumski biomarker in možnosti farmakološke modulacije
Podobna dela
Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:
Nazaj