Multiscale spatial segregation analysis in digital images of biofilms

Dogša, Iztok (avtor), Mandić-Mulec, Ines (avtor)

Quantifying the degree of spatial segregation of two bacterial strains in mixed biofilms is an important topic in microbiology. Spatial segregation is dependent on spatial scale as two strains may appear to be well mixed if observed from a distance, but a closer look can reveal strong separation. Typically, this information is encoded in a digital image that represents the binary system, e.g., a microscopy image of a two species biofilm. To decode spatial segregation information, we have developed quantitative measures for evaluating the degree of the spatial scale-dependent segregation of two bacterial strains in a digital image. The constructed algorithm is based on the new segregation measures and overcomes drawbacks of existing approaches for biofilm segregation analysis. The new approach is implemented in a freely available software and was successfully applied to biofilms of two strains and bacterial suspensions for detection of the different spatial scale-dependent segregation levels.

• Jezik: Angleški jezik
• Ključne besede: microbiology, bacterial strains, biofilm, spatial segregation, biofilm analysis
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 12 str.
• Številčenje: Vol. 6, art. 100157
• PID: 20.500.12556/RUL-152886
• UDK: 579.2
• ISSN pri članku: 2590-2075
• DOI: 10.1016/j.bioflm.2023.100157
• COBISS.SI-ID: 173842691
• Datum objave v RUL: 11.12.2023

Gradivo je del revije

• Naslov: Biofilm
• Založnik: Elsevier
• ISSN: 2590-2075
• COBISS.SI-ID: 5030264

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikrobne združbe, biofilm, prostorska razporeditev, analiza biofilma