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Expression of GFP and DsRed fluorescent proteins after gene electrotransfer of tumour cells in vitro
ID
Komel, Tilen
(
Avtor
),
ID
Bošnjak, Maša
(
Avtor
),
ID
Serša, Gregor
(
Avtor
),
ID
Čemažar, Maja
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(4,08 MB)
MD5: FB9CAE4D8FD1EF89E111E79F4F391800
URL - Izvorni URL, za dostop obiščite
https://www.sciencedirect.com/science/article/pii/S1567539423001275
Galerija slik
Izvleček
Fluorescent reporter genes are widely used to study the transfection of various types of primary cells and cell lines. The aim of our research was to investigate the expression dynamics of GFP and DsRed reporter genes individually and combined after gene electrotransfer of plasmids with two different electroporation protocols in B16F10 and CT26 cells in vitro. The cytotoxicity after gene electrotransfer of both plasmids was first determined. Second, the intensity of fluorescence and the percentage of cells transfected with both plasmids individually and in combination were monitored in real time. The results show that the percentage of viability after gene electrotransfer of plasmids using the EP2 pulses was significantly higher compared to the EP1 pulses. In contrast, the percentage of transfected cells and fluorescence intensity were higher after gene electrotransfer with the EP1 pulse protocol. Moreover, the percentage of transfected cells was higher and started earlier in the B16F10 cell line than in the CT26 cell line. However, fluorescence intensity was higher in CT26 cells. Co-expression of fluorescent proteins was achieved only in a small number of cells. In conclusion, this study elucidated some of the dynamics of reporter gene expression in cancer cell lines after gene electrotransfer.
Jezik:
Angleški jezik
Ključne besede:
B16F10
,
CT26
,
gene electrotransfer
,
concomitant plasmid delivery
,
reporter proteins
,
real-time monitoring
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
ZF - Zdravstvena fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2023
Št. strani:
12 str.
Številčenje:
Vol. 153, art. 108490
PID:
20.500.12556/RUL-152846
UDK:
602.6/.7
ISSN pri članku:
1878-562X
DOI:
10.1016/j.bioelechem.2023.108490
COBISS.SI-ID:
159032323
Datum objave v RUL:
08.12.2023
Število ogledov:
355
Število prenosov:
47
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Bioelectrochemistry
Založnik:
Elsevier
ISSN:
1878-562X
COBISS.SI-ID:
23407109
Licence
Licenca:
CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:
Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
B16F10
,
CT26
,
elektroprenos genov
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P3-0003
Naslov:
Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J3-2528
Naslov:
Imunski genski elektroprenos IL-12 in IL-2 za zdravljenje immunološko hladnih/vročih tumorjev
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