20.500.12556/RUL-152846 Expression of GFP and DsRed fluorescent proteins after gene electrotransfer of tumour cells in vitro Fluorescent reporter genes are widely used to study the transfection of various types of primary cells and cell lines. The aim of our research was to investigate the expression dynamics of GFP and DsRed reporter genes individually and combined after gene electrotransfer of plasmids with two different electroporation protocols in B16F10 and CT26 cells in vitro. The cytotoxicity after gene electrotransfer of both plasmids was first determined. Second, the intensity of fluorescence and the percentage of cells transfected with both plasmids individually and in combination were monitored in real time. The results show that the percentage of viability after gene electrotransfer of plasmids using the EP2 pulses was significantly higher compared to the EP1 pulses. In contrast, the percentage of transfected cells and fluorescence intensity were higher after gene electrotransfer with the EP1 pulse protocol. Moreover, the percentage of transfected cells was higher and started earlier in the B16F10 cell line than in the CT26 cell line. However, fluorescence intensity was higher in CT26 cells. Co-expression of fluorescent proteins was achieved only in a small number of cells. In conclusion, this study elucidated some of the dynamics of reporter gene expression in cancer cell lines after gene electrotransfer. B16F10 CT26 gene electrotransfer concomitant plasmid delivery reporter proteins real-time monitoring B16F10 CT26 elektroprenos genov true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-08 16:07:20 2023-12-08 16:07:22 2023-12-09 03:49:42 0000-00-00 00:00:00 2023 0 0 12 str. Vol. 153, art. 108490 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.6/.7 1878-562X 10.1016/j.bioelechem.2023.108490 159032323 1-s2.0-S1567539423001275-main.pdf 1-s2.0-S1567539423001275-main.pdf 1 FB9CAE4D8FD1EF89E111E79F4F391800 2b742e23eecd76eb5bd54d88bc274d7d584c389dfa6dce07ee390c8874bc7b35 f0b23425-95db-11ee-9c20-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178541 https://www.sciencedirect.com/science/article/pii/S1567539423001275 0 c55c5e08-95db-11ee-9c20-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178540 Medicinska fakulteta Zdravstvena fakulteta 0 0 0